家兔胚胎胃肠道细菌分离及16S rRNA基因序列分析  

Isolation and 16S rRNA gene sequence analysis of gastrointestinal bacteria in the rabbit embryos

作　　者：郭海勇[1] 单晓枫[2] 吴静[2] 孙文怡[1] 于超[2] 王好[2] 钱爱东[2] 

机构地区：[1]吉林师范大学生命科学学院,吉林四平136000 [2]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国预防兽医学报》2012年第12期967-971,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30871875)

摘　　要：为研究家兔胚胎胃肠道细菌菌群的种类,对常规饲养、正常妊娠的家兔胚胎后期胃肠道细菌进行分离,基于分离细菌的生理生化特性、16S rRNA基因序列的同源性比较及系统发育分析,初步确定家兔胚胎后期胃肠道中存在的微生物菌群有微球菌属(Micrococcus)、葡萄球菌属(Staphylococcus)、棒杆菌属(Corynebacterium)以及蜡样芽孢杆菌(Bacillus cereus)等细菌,其序列同源性均为97%～99%。分离细菌均为G+细菌,家兔胚胎胃肠道中细菌的平均数量达到0.8×102cfu/g～1.2×102cfu/g,本研究为哺乳动物胚胎时期存在于胃肠道中细菌的深入研究奠定了基础。In order to study the variety of gastrointestinal bacteria in the rabbit embryos, the bacteria of gastrointestinal tract in the later embryonic period were isolated from the conventional feeding and normal pregnant rabbits. Based on the bacterial physiological and biochemical properties, homology and phylogenetic analysis of I6S rRNA gene sequence, four isolates were identified as the genera Micrococcus, Staphylococcus, Corynebacterium and Bacillus cereus, all of which have 97% to 99% similarity. The bacteria were the gram-positive bacterium and the average number of bacteria was 0.8×10^2 cfu/g to 1.2×10^2 cfu/g in gastrointestinal tract of the rabbit embryo in the later embryonic period. This study would lay a foundation for further studies on bacteria of gastrointestinal tract from the mammalian embryo stage.

关 键 词：家兔胚胎 胃肠道 细菌分离 16S RRNA基因序列 

分 类 号：S852.61[农业科学—基础兽医学]