奶牛边缘无浆体MSP2的原核表达及抗原性鉴定  

Expression and immunogenicity analysis of membrane surface protein 2 of Anaplasma marginale

作　　者：邵光喜[1,2] 马国林 衣菲[1] 郎秀艳[1] 蒋慧婷[1] 王春仁[1] 刘娇[1] 田秋丰[1] 倪宏波[1] 钱爱东[2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]吉林农业大学动物科技学院,吉林长春130118 [3]黑龙江省鹤山农场鹤澳奶牛繁育中心,黑龙江齐齐哈尔161443

出　　处：《中国预防兽医学报》2012年第12期999-1001,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：研究生创新科研项目(YJSCX2011-278HLJ);吉林省科技发展计划项目(20100220);黑龙江省教育厅骨干教师项目(1251G044);黑龙江省教育厅畜禽健康养殖技术体系创新团队项目(2010td05)

摘　　要：为克隆和原核表达牛边缘无浆体(A.marginale)膜表面蛋白2(MSP2),并分析其免疫原性,本研究通过PCR方法扩增A.marginale的MSP2基因,将其连接到pGEX 6p-1载体中,转化感受态细胞BL21(DE3),经IPTG诱导表达,进行SDS-PAGE分析,将纯化的MSP2重组蛋白免疫小鼠。结果表明表达并纯化了A.marginaleMSP2,其免疫原性良好,为制备单克隆抗体及亚单位疫苗的研制奠定了基础。To express the membrane surface protein 2 （MSP2） of Anaplasma marginale and identify its immunogenicity. The MSP2 gene of A.marginale was amplified by PCR and cloned into pGEX-6P-1 vector for expression in E.coli. SDS-PAGE analysis showed that the recombinant protein was about 68 ku, which reacted positively with anti- A.marginale serum detected by western blot. The expression of A.marginale MSP2 provided a basis for monoclonal antibody and subunit vaccine development against A.marginale.

关 键 词：边缘无浆体 MSP2 原核表达 免疫原性 

分 类 号：S852.64[农业科学—基础兽医学]