大熊猫隐孢子虫的分离鉴定  

Isolation and identification of Cryptosporidiumfrom giant panda

作　　者：刘学涵[1] 何廷美[1,2] 钟志军[1] 王承东[2] 张和民[2] 李德生[2] 何永果[2] 李才武[2] 邓家波[3] 王强 彭广能[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]中国林业科学研究院大熊猫研究中心四川雅安碧峰峡基地,四川雅安625007 [3]成都动物园,四川成都610014

出　　处：《中国兽医科学》2012年第11期1107-1111,共5页Chinese Veterinary Science

基　　金：大熊猫国际合作资金项目(SD0632);教育部“长江学者和创新团队发展计划”创新团队项目(IRT0848);成都大熊猫繁育研究基金会项目(CPF申2012-01)

摘　　要：为了探讨大熊猫隐孢子虫的感染情况,用饱和蔗糖溶液漂浮法检测来自大熊猫研究中心的57份大熊猫粪样,并对阳性分离株进行形态学、分子生物学鉴定和系统发育树构建。结果显示,在1只大熊猫(雄性,18岁)粪样中发现了隐孢子虫,卵囊的平均大小为4.60μm×3.99μm;在其18SrRNA、HSP70位点上,该分离株与隐孢子虫黑熊基因型同源性分别为98.5%、99.5%,碱基差异分别有12个和10个;种系发育显示宿主适应性特点。建议将此分离株定为新的基因型——隐孢子虫大熊猫基因型。To investigate the prevalence of Cryptosporidiurn infected giant panda,a total of 57 giant panda＇s fecal specimens were collected from China Conservation and Research Center for the Giant Panda in Sichuan Province,China. The specimens were examined for Cryptosporidium oocysts by the sheather＇s sugar flotation technique and the oocysts were identified with morphology, molecular characterization and phylogenetic analysis. The results showed that an adult giant panda（male, 18 years-old） was infected by Cryptosporidiurn. In morphology, the average size of the oocysts was 4. 60 μm × 3. 99 μm. At the 18 S rRNA and HSP70 loci,the homologies of the isolate to black bear genotype were 98.5 % and 99.5 %, respectively,with 12 and 10 nucleotide differences. In addition,the isolate displayed host adaptation. There- fore, the Cryptosporidium in giant panda in this study is proposed as a new genotype, the Cryptosporidium giant panda genotype.

关 键 词：隐孢子虫 大熊猫 聚合酶链反应 基因型 

分 类 号：S852.723[农业科学—基础兽医学]