成年大熊猫肠道菌群多样性的16S rDNA-RFLP分析  

Research on diversity of intestinal flora in an adult giant panda based on the 16S rDNA-RFLP

作　　者：何廷美[1,2] 崔婷婷[1,3] 钟志军[1] 王承东[2] 何永果[2] 王燚[1] 张和民[2] 李德生[2] 李才武[2] 张婷婷[1] 罗永久[1] 程安春[1] 彭广能[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]中国林业科学研究院大熊猫研究中心四川雅安碧峰峡基地,四川雅安625007 [3]德阳市畜牧食品局,四川德阳618000

出　　处：《中国兽医科学》2012年第11期1121-1127,共7页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(31272620);大熊猫国际合作资金项目(SD0632);四川省2011年学术和技术带头人培养资金资助项目;教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848);四川省科技厅科技支撑项目(2011NZ0060);四川农业大学双支计划项目(00270706;00270713)

摘　　要：为探究秋季(9～11月)成年大熊猫肠道菌群的多样性,以细菌16SrDNA通用引物扩增粪样总细菌16SrDNA基因并构建文库,然后采用16SrDNA基因限制性片段长度多态性(restriction fragmentlength polymorphism,RFLP)技术对成年大熊猫秋季肠道菌群多样性进行分析。结果显示,大肠杆菌、假单胞菌、链球菌和鞘氨醇杆菌为大熊猫秋季肠道内的优势菌群,肠道内细菌多样性9月较高,11月较低。结果表明,16SrDNA-RFLP技术能用于成年大熊猫肠道菌群多样性的研究。To investigate the diversity of intestinal bacteria of giant pandas in Autumn（September to November）, the 16 S rDNA of total bacteria was amplified with bacterial universal primer of 16 S rDNA and the 16 S rDNA clone libraries were constructed. The diversity of 16 S rDNA clone library was analyzed by 16 S rDNA-restriction fragment length polymorphism（RFLP） technique. Results showed that the dominant microflora in giant panda＇s intestine were Escherichia coli, Pseudomonas, Streptococcus and Sphingobacterium. The diversity of bacteria in the intestinal tract of giant panda in September was higher than that in November. Results demonstrated that the technology based on 16 S rDNA-RFLP can be used to the study on diversity of intestinal flora in giant panda.

关 键 词：大熊猫 肠道菌群 多样性 16S RDNA 限制性片段长度多态性 

分 类 号：S852.61[农业科学—基础兽医学]