双表达重组腺病毒介导的骨形态发生蛋白2-9复合纳米羟基磷灰石/聚酰胺66融合器用于山羊椎间融合的效果评价  

Evaluation on effects of bone morphoge netic protein2-9 mediated by co-expressing recombinant adenovirus combined with nano-hydroxyapatite/polyamide 66 cage on lumbar interbody fusion of goats

作　　者：马金梁[1] 黄帆[1] 汪洋[1] 罗进勇[2] 邓忠良[1] 

机构地区：[1]重庆医科大学附属第二医院骨科,重庆400010 [2]重庆医科大学分子医学实验室,重庆400016

出　　处：《吉林大学学报（医学版）》2012年第6期1086-1090,共5页Journal of Jilin University：Medicine Edition

基　　金：国家"863"计划资助课题(2008AA02Z443);重庆市卫生局科研基金资助课题(2011-1-053)

摘　　要：目的:比较双表达重组腺病毒介导的骨形态发生蛋白2-9(Adv-BMP2-9)与单表达Adv-BMP9和Adv-BMP2复合纳米羟基磷灰石/聚酰胺66(nHA/PA66)融合器用于山羊椎间融合的效果,验证BMP2与BMP9的协同成骨作用。方法:选用40只中国青山羊行L2-3、L3-4和L4-5腰椎间融合术,共融合120个腰椎间隙。每个腰椎间隙植入nHA/PA66融合器复合以下4种腺病毒之一,Adv-BMP9(Adv-BMP9组,n=30)、Adv-BMP2(Adv-BMP2组,n=30)、Adv-BMP2-9(Adv-BMP2-9组,n=30)和Adv-GFP(Adv-GFP组,n=30)。术后24周处死所有山羊,取出融合节段标本,行影像学评估和生物力学分析。结果:X线检测,术前、术后各组间椎间隙高度(DSH)比较差异无统计学意义(P>0.05);各组标本椎间融合率,Adv-BMP2-9组为70%(21/30),Adv-BMP9组为53%(16/30),Adv-BMP2组为43%(13/30),而Adv-GFP组为30%(9/30)。各组椎间CT融合度评分,Adv-hBMP2-9组、Adv-hBMP9组、Adv-hBMP2组和Adv-GFP组依次为52.29±0.72、44.02±0.67、38.83±0.86和28.65±0.47,各组间比较差异均具有统计学意义(P<0.05或P<0.01)。生物力学评估,Adv-BMP2-9组融合节段刚度大于Adv-BMP9组(P<0.05);Adv-BMP9组融合节段刚度大于Adv-BMP2组,但差异无统计意义(P>0.05)。实验期间各组山羊均未观察到局部或远处感染、发热和异物排斥反应。结论:双表达Adv-BMP2-9复合nHA/PA66融合器对山羊椎间融合效果优于单表达Adv-BMP9或Adv-BMP2,BMP2与BMP9具有协同成骨作用。Objective To compare the interbody fusion effects of bone morphogenetic protein 2-9（Adv-BMP 2-9） with single expression of Adv-BMP9 or adv-BMP2 mediated by co-expressing recombinant adenovirus combined with nano-hydroxyapatite/polyamide 66（nHA/PA66） cage on lumbar interbody fusion of goats,and to clarify the collaborative osteogenesis effects of BMP2 and BMP9.Methods Interbody fusion was performed at L2-3,L3-4 and L4-5 in 40 Chinese green goats,included a total of 120 interspace of lumbar vertebrae.Each interspace of lumbar vertebra was randomly implanted with a nHA/PA66 cage combined with one of the following four adenovirus,Adv-BMP9（Adv-BMP9 group,n=30）,Adv-BMP2（Adv-BMP2 group,n=30）,Adv-BMP2-9（Adv-BMP2-9 group,n=30）,and Adv-GFP（Adv-GFP group,n=30）.The goats were euthanized 24 weeks after operation,and the fusion segment speciments were obtained;radiographic inspection and biomechanical analysis were performed on the segment speciments.Results The X-ray results showed there was no significant difference of disc space height（DSH） before and after operation between various groups.The interbody fusion rates were 70%（21/30） in Adv-BMP2-9 group and 53%（16/30） in Adv-BMP9 group and 31%（5/16） in Adv-BMP2 group and 30%（9/30） in Adv-GFP group.CT fusion degree tests showed that CT fusion scores in Adv-hBMP2-9 group,Adv-hBMP9 group,Adv-hBMP2 group and Adv-GFP group were 52.29±0.72,44.02±0.67,38.83±0.86,and 28.65±0.47,respectively;there were significant differences between various groups（P0.05 or P0.01）.Biomechanical analysis showed that the stiffness of the fusion segment in Adv-BMP2-9 group was higher than that in Adv-BMP9 group（P0.05）,and stiffness in the fusion segment in Adv-BMP9 group was higher than that in Adv-BMP2 group,but there was no significant difference（P0.05）.During the experiment,no local or distant infection,fever,and foreign body rejection were observed in all goats.Conclusion The interbody fusion effect of co-expressing Adv-BMP2-9 combined with

关 键 词：骨形态发生蛋白 重组腺病毒 椎间融合 纳米羟基磷灰石 聚酰胺 

分 类 号：R681.5[医药卫生—骨科学]