绞股蓝总皂苷对光老化HaCaT细胞中p38MAPK蛋白和c-Jun mRNA表达的抑制作用  

Inhibitory effect of gypenosides on expressions of p38MAPK protein and c-Jun mRNA in photoaging HaCaT cells

作　　者：陶冶[1] 张小卿[2] 吴琼[3] 吴景东[4] 

机构地区：[1]辽宁中医药大学基础医学院中医基础教研室,辽宁沈阳110847 [2]辽宁中医药大学针灸推拿学院针灸教研室,辽宁沈阳110847 [3]中国医科大学附属盛京医院小儿神经内科,辽宁沈阳110004 [4]辽宁中医药大学学生工作处,辽宁沈阳110847

出　　处：《吉林大学学报（医学版）》2012年第6期1147-1151,共5页Journal of Jilin University：Medicine Edition

基　　金：辽宁省科技厅自然科学基金资助课题(20102149)

摘　　要：目的:观察绞股蓝总皂苷含药血清对光老化HaCaT细胞p38促分裂原活化蛋白激酶(p38MAPK)蛋白和Jun基因(c-Jun)mRNA表达的影响,探讨绞股蓝总皂苷含药血清抗皮肤光老化的作用机制。方法:采用中波紫外线(UVB)照射建立光老化HaCaT细胞模型,加低、中、高剂量绞股蓝总皂苷含药血清于光老化HaCaT细胞模型培养液中培养,并设空白对照组、空白血清组和UVB模型组。采用Western blotting和RT-PCR方法检测各组细胞p38MAPK蛋白和c-Jun mRNA的表达水平。结果:与空白对照组比较,UVB模型组细胞p38MAPK蛋白和c-Jun mRNA表达显著上调,差异有统计学意义(P<0.01);与UVB模型组比较,低、中和高剂量含药血清组细胞p38MAPK蛋白和c-Jun mRNA表达显著下调,差异有统计学意义(P<0.01);与空白血清组比较,低、中和高剂量含药血清组细胞p38MAPK蛋白和c-Jun mRNA表达下降(P<0.01)。结论:绞股蓝总皂苷含药血清可能通过抑制p38MAPK蛋白和c-Jun mRNA表达抑制皮肤光老化。Objective To observe the effect of gypenosides-containing serum on the expressions of p38 mitogen-activated protein kinase（p38MAPK）protein and c-Jun mRNA in photoaging HaCaT cells and to explore the mechanism of inhibitory effect of gypenosides-containing serum on photoaging.Methods The photoaging HaCaT cell model was established by UVB radiation,low,middle,and high doses of gypenosides-containing serum were used in the culture fluid of phatoaging HaCaT cell models;meanwhile blank control group,blank serum group and UVB model group were set up.The expression levels of p38MAPK protein and c-Jun mRNA of cells in each group were measured by Western blotting and RT-PCR methods.Results Compared with blank control group,the expression levels of p38MAPK protein and c-Jun mRNA of the cells in UVB model group were significantly increased,the difference had statistical significance（P0.01）.Compared with UVB model group,the expression levels of p38MAPK protein and c-Jun mRNA of cells in low,middle and high doses of gypenosides-containing serum groups were significantly decreased,the difference had statistical significance（P0.01）.Compared with blank serum group,the expression levels of p38 MAPK protein and c-Jun mRNA of the cells in low,middle and high doses of gypensides-containing serum groups were decreased（P0.01）.Conclusion Gypenosides-containing serum could prevent the skin photoaging by inhibinting the expressions of p38MAPK protein and c-Jun mRNA.

关 键 词：光老化 绞股蓝总皂苷 角质形成细胞 中波紫外线 p38促分裂原活化蛋白激酶 Jun基因 

分 类 号：R758.14[医药卫生—皮肤病学与性病学]