人工异源六倍体小麦部分同源基因的表达变化  

Expression of Homoelogous Gene in Synthetic Hexaploid Wheat

作　　者：王延召[1] 聂利红[1] 孙其信[2] 胡学安[1] 魏良明[1] 周波[1] 

机构地区：[1]河南省农业科学院,河南郑州450002 [2]西北农林科技大学,陕西杨凌712100

出　　处：《河南农业科学》2012年第11期19-21,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30370878)

摘　　要：为了研究小麦从四倍体到六倍体进化过程中部分同源基因的表达变化,以人工异源六倍体小麦SCA/SQ及其亲本SCAUP和SQ523为材料,采用cDNA-SSCP方法分析了208个部分同源基因不同拷贝的表达情况。结果表明,有10个来自父本的拷贝在人工异源六倍体小麦中沉默,进一步分析了这些沉默表达的基因,发现其中有5个是由于在DNA序列上丢失,而另外5个可能是由于甲基化或者其他机制引起的。In order to understand the homoeologous gene changes during the evolution of hexaploid wheat from tetroploid one, the artificial synthesized heterogenous hexaploid wheat SCA/SQ and its parents SCAUP and SQ523 were taken as materials to study the homoelogous gene expression by means of cDNA-SSCP analysis. Results showed that 10 gene copies from male parent SQ523 were silenced in synthetic hexaploid wheat, among which 5 copies were caused by the loss of corresponding DNA sequences, and other 5 ones were due to methylation or other mechaniisms according to the DNA-SSCP analysis.

关 键 词：人工异源六倍体小麦 多倍化 基因表达变化 

分 类 号：S512.1[农业科学—作物学]