应用相互嫁接技术研究棉花对黄萎病的抗性  

Use of Reciprocal Grafting to Study Verticillium Wilt Resistance of Cotton

作　　者：胡雨薇[1,2,3] 郝俊杰[2] 贾新合[4] 马奇祥[2] 崔小伟[2] 高俊山 李宾[4] 郭利娟 王庆东[1] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]河南省农业科学院植物保护研究所,农业部华北南部农作物有害生物综合治理重点实验室/河南省农作物病虫害防治重点实验室,河南郑州450002 [3]新乡医学院三全学院,河南新乡453003 [4]郑州市农林科学研究所,河南郑州450005 [5]开封市农林科学研究院,河南开封475004 [6]禹州市农业林业局,河南禹州461670

出　　处：《河南农业科学》2012年第11期88-91,共4页Journal of Henan Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2008ZX08005-002);国家棉花现代农业产业技术体系项目(CARS-18)

摘　　要：选用对棉花黄萎病表现不同抗性的棉花材料,应用相互嫁接的方法将抗病和感病棉花品种组合在一起构建嫁接系统,将其种植在连作多年的棉田,调查棉花黄萎病的发生情况。结果表明,自身嫁接苗与自根苗抗性差异不显著;相互嫁接植株抗/感(接穗/砧木,下同)和感/抗组合抗病效果明显强于感/感组合,但较抗/抗组合抗病性下降。研究认为,抗病材料无论作砧木还是接穗,均能抑制黄萎病的发生,说明抗病材料植株整体对棉花黄萎病菌具有抵抗能力。In an attempt to better understand the resistance mechanism of cotton to Verticillium wilt,cotton lines with different resistance levels to Verticillium wilt were selected,and reciprocal grafting were made between resistant and susceptible genotypes. The grafted combinations were planted in the naturally infected cotton field, and the data of cotton Verticillium wilt were investi-gated. The results indicated that the resistance level was not significantly different between self-root cotton and their self-grafted cotton. The resistance of resistant/susceptible and susceptible/ resistant grafted combinations was higher than that of susceptible/susceptible combination, but lower than that of resistant/resistant combination. Resistant cotton genotypes either as stock or scion can inhibit Verticillium wilt,which indicates that the whole plant of resistant cotton can re-sist to Verticillium wilt.

关 键 词：棉花 黄萎病 相互嫁接 抗性 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]