正交设计法优化蒲公英中黄酮提取工艺  

Optimization of the extracting process for flavonoid from Herba Taraxaci by orthogonal design

作　　者：陈虹[1] 叶得河[1] 杨明[1] 王桂荣[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中兽医医药杂志》2012年第6期40-42,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省技术研究与开发专项计划项目(QS031-C3-11)

摘　　要：在510 nm最大吸收光时检测正交提取蒲公英中的黄酮含量,建立正交提取工艺,对提取物进行HPLC检测,色谱柱为Eclipse×DB-C18柱(4.6 mm×250 mm,5μm),流动相为甲醇∶1%冰醋酸=60∶40(v/v),流速1.0 mL/min,检测波长254 nm,柱温30℃,进样量20μL,在此色谱条件下测定黄酮含量。结果表明,各因素影响力大小为:pH值>浸泡时间>料液比;用HPLC测定蒲公英中的黄酮含量为9.26%。Orthogonal extraction process for flavonoid from Herba Taraxaci was established by orthogonal test in 510 nm maximum optical absorption; Then the extract was detected by HPLC. The chromatographic condition was as follows： analytic column： Eclipse ×DB-C18 （4.6 mm × 250 mm, 5 μm）, the mobile phase was methanol 1：1% glacial acetic acid = 60：40 （v/v）, the flow rate was 1.0 mL/min, UV detection wavelength was 254 nm, column temperature was 30 ℃, aud sample size was 20 μL. The results showed that the factors influence was： pH value 〉 exlracting time 〉 solid to liquid ratio： the maximal flavonoid content in Herba Taraxaci was 9.26% detected by HPLC.

关 键 词：蒲公英 黄酮 正交设计 

分 类 号：S853.72[农业科学—临床兽医学]