原生质体紫外诱变选育竹红菌甲素高产菌株  

Screening of high-yield hypocrellin A producing mutants from Shiraia sp.S8 by protoplast mutagenesis and ultraviolet irradiation

作　　者：潘魏松[1] 嵇源源[1] 杨珠英[1] 王剑文[1] 

机构地区：[1]苏州大学药学院,苏州215123

出　　处：《生物加工过程》2012年第6期18-23,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家教育部留学基金资助项目(K513201011);苏州大学东吴学者计划资助项目(14317363);江苏省六大人才高峰项目(Bu132802);国家大学生创新性实验计划资助项目

摘　　要：采用原生质体紫外诱变技术选育竹红菌甲素高产菌株。结果表明:以竹黄菌Shiraia sp.S8为出发菌株,当使用混合酶系(5 mg/mL纤维素酶和10 mg/mL蜗牛酶)在30℃处理菌丝2 h,获得菌丝原生质体3.24×106个/mL。以竹黄菌原生质体在距离15 W紫外灯30 cm处照射诱导,获得诱变菌株C6。其竹红菌甲素产量达到28.1 mg/L,比原始出发菌株提高了53.7%,且遗传稳定,具有较高的医药与工业应用价值。A Shiraia sp. S8 mutant strain with higher yield of hypocrellin A was screened. Then, optimum conditions for protoplast preparation （3.24×10^6 mL-1） were determined. After 50 h cultivation the hy-pha were collected and digested with the mixing enzymes including cellulase （5 mg/mL） and snailase （ 10 mg/mL） for 2 h at 30℃. The C6 mutant with higher hypocrellin A yield was obtained via ultraviolet irradiation （30 cm from a 15 W UV lamp） on the isolated protoplasts. The yield of hyprocrellin A （28.1 mg/L） by mutant C6 was increased by 53.7% than that of the start strain S8. The production of the mu- tant was verified to be genetically stable.

关 键 词：竹黄菌 原生质体诱变 竹红菌素 选育 

分 类 号：Q784[生物学—分子生物学]