渗透压调控对裂殖壶菌发酵产DHA的影响  

Fermentation regulation of DHA production by Schizochytrium sp. based on osmotic pressure

作　　者：曾艳[1] 冯云[1] 仝倩倩[2] 任路静[1] 黄和[1] 

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京210009 [2]淮南师范学院,淮南232001

出　　处：《生物加工过程》2012年第6期24-28,共5页Chinese Journal of Bioprocess Engineering

基　　金：教育部新世纪优秀人才支持计划资助项目(NCET-09-0157);霍英东教育基金资助项目(123014);江苏省高校产业化推进项目(JH09-30);江苏省六大高峰人才项目(2008-D-63)

摘　　要：考察了不同渗透胁迫(0、10、20、30和40 g/L NaCl)对裂殖壶菌HX-308发酵产DHA及脂肪酸构成的影响。结果表明:20 g/L NaCl最有利于裂殖壶菌生长和DHA积累,生物量、总脂肪酸含量、DHA产量及DHA占生物量的比值分别为73 g/L、10.7 g/L、5.0 g/L和68 mg/g,并且DHA在总脂肪酸中所占百分比最高,为45.2%。此外,在低渗透压(10 g/L NaCl)条件下,添加40 mmol/L甘氨酸甜菜碱,DHA产量与未添加相比提高了28.21%;在高渗透压(40 g/L NaCl)条件下添加40 mmol/L海藻糖,DHA产量提高了46.84%;表明添加适量的外源相容性溶质能有效地促进裂殖壶菌积累DHA。The effects of different osmotic pressures （0,10,20,30 and 40 g/L NaC1） on DHA accumula- tion and fatty acids composition of Schizochytrium sp. HX-308 were studied. 20 g/L salinity was proved to be beneficial for cell growth and DHA production. Cell dry weight （DCW） , total content of fatty acids,DHA yield, and DHA concentration on DCW were the highest, around 73 g/L, 10.7 g/L, 5.0 g/L, and 68 mg/g, respectively. The DHA percentage of total fatty acids also reached the maxi- mum of 45.2 %. In addition, under the conditions of 40 mmol/L glyeine betaine addition under low sa- linity （ 10 g/L NaCl） and 40 mmol/L trehalose addition under high salinity （40 g/L NaC1） DHA yield could increase by 28.21% and 46. 84% , respectively, thus demonstrating the positive effect of compatible solute addition to be effective for DHA production by Schizochytrium sp.

关 键 词：裂殖壶菌 DHA 渗透压 相容性溶质 

分 类 号：Q815.1[生物学—生物工程]