化学衍生-溶剂结晶法从植物甾醇混合组分中分离豆甾醇  

Separation of stigmasterol from phytosterol mixture by chemical derivatization and solvent crystallization

作　　者：杨建洪 杨顺楷[2] 曾辉[3] 吴中柳[2] 

机构地区：[1]四川大学华西临床医学院,成都610041 [2]中国科学院成都生物研究所,成都610041 [3]成都大学生物产业学院,成都610000

出　　处：《生物加工过程》2012年第6期38-41,共4页Chinese Journal of Bioprocess Engineering

基　　金：成都生物研究所生物催化与分子进化实验室基金资助项目

摘　　要：采用化学衍生和溶剂结晶法,建立起一条从植物甾醇混合组分分离豆甾醇的技术途径。通过对植物甾醇的乙酰化,溴化-脱溴和皂化反应,并结合相应试样的重结晶操作以分离豆甾醇。利用此方法从植物甾醇混合组分分离豆甾醇,其产率为6.9%,回收率45.7%,具有生物工业应用潜力。To develop a process for the separation of stigmasterol from phytosterol mixture by chemical derivatization and solvent crystallization. Its performance procedure, including acetylation, bromization, debromination, and saponification of phytosterol mixture, and crystallization with solvent, was used at the gramme level on a test bench. The yield of stigmasterol is 6.9% and the recovery rate is 45.7%. The phytosterol mixture was separated by the method. This illustrate that the method was feasible and suitable for a pilot run.

关 键 词：植物甾醇混合组分 化学衍生 溶剂结晶 豆甾醇 

分 类 号：TQ654.98[化学工程—精细化工]