浙贝乙素胆酸盐中浙贝乙素和胆酸的含量测定  

Determination of Verticinone and Cholic Acid in Verticinone Cholic Acid Salt by HPLCELSD

作　　者：李娟[1] 张鹏[2] 钟淑文[2] 皮慧芳[2] 阮汉利[2] 吴继洲[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030 [2]华中科技大学同济医学院药学院天然药物化学与资源评价湖北省重点实验室,武汉430030

出　　处：《医药导报》2012年第12期1616-1618,共3页Herald of Medicine

基　　金：湖北省自然科学基金资助项目(2008CDA046)

摘　　要：目的建立浙贝乙素胆酸盐中浙贝乙素和胆酸的含量测定方法。方法以甲醇-乙酸铵(0.1 mol.L-1)-三乙胺-冰乙酸(78∶22∶0.2∶0.03)为流动相,C18柱(250 mm×4.6 mm,5μm)分离,流速为1 mL.min-1;蒸发光散射检测器检测(漂移管温度72.1℃,氮气流速2 mL.min-1)。结果浙贝乙素胆酸盐中浙贝乙素和胆酸分别在50.4～151.1μg.mL-1(r=0.999 6,n=5)、50.5～151.4μg.mL-1(r=0.999 9,n=5)内峰面积与浓度呈良好线性关系;平均回收率分别为99.17%(RSD=0.62%)和99.37%(RSD=0.63%)。结论该方法测定浙贝乙素胆酸盐中的浙贝乙素和胆酸含量简便,可靠,重复性好。Objective To establish an HPLC method for determination of verticinone and cholic acid in verticinone- cholic acid salt. Methods The separation was performed on a C18column （250 mm×4.6 mm,5 μm）. The mobile phase was methanol ： ammonium acetate （0.1 mol· L-l） ： triethylamine ： ethanoic acid （78： 22 ： 0.2 ： 0.03）. The flow rate was 1 mL·min-. The gas flow rate of ELSD was 2 mL · min-1 and the temperature of drift tube was 72.1℃. Results The linear ranges of vertieinone and cholic acid were 50.4-151.1μg · mL-l（ r = 0. 999 6, n = 5 ） and 50.5-151.4 μg · mL-l（ r = 0. 999 9,n = 5） respectively. The average recoveries of the two components were 99.37% （ RSD = 0.63% ,n = 9） and 99.17% （ RSD = 0.62%, n = 9 ） respectively. Conclusion The method is simple and reliable for the quality control of verticinone and cholieacid salt.

关 键 词：浙贝乙素胆酸盐 浙贝乙素 胆酸 高效液相-蒸发光散射 含量测定 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]