2010年-2011年我国五省PRRSV分离株ORF5基因遗传变异分析  

Genetic Variation Analysis of ORF5 Genes of PRRSV Isolates in Five Provinces of China in 2010-2011

作　　者：刘沫飞[1] 李蕾[1] 郑辉[2] 李岚[3] 王赛赛[4] 吴发兴[2] 李晓成[2] 王洪斌[1] 黄保续[2] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150036 [2]中国动物卫生与流行病学中心,山东青岛266000 [3]云南农业大学,云南昆明650201 [4]福建农林大学,福建福州350002

出　　处：《动物医学进展》2012年第12期6-11,共6页Progress In Veterinary Medicine

基　　金：国家十一五科技支撑计划项目(2007BAD86B05)

摘　　要：为了解2010年-2011年间我国5省猪繁殖与呼吸综合征病毒(PRRSV)流行毒株的分子生物学特性及变异规律,应用Mcar-145细胞分离得到15株PRRSV,通过RT-PCR方法对15株PRRSV的ORF5基因进行扩增和序列分析。依据NCBI所下载的标准序列设计一对引物,对ORF5全长进行扩增(603bp),分离株之间的核苷酸同源性为98.8%～99.9%,核苷酸推导的氨基酸同源性为95.5%～99.5%。15株PRRSV分离株与美洲型的PRRSV同源性较高,可推断均属美洲型。其中9株ORF5(151)氨基酸位点发生与HB-1相同的变异,即R151→K151,其余6株ORF5(151)氨基酸位点与JAX1相同,即ORF5(151)为R。系统进化树表明,BFLN3-2010、BFSD1-2011和BFAH2-2011 3株与CH-1a在同一分支,属于第2亚群,BFLN2-2010等12株与JAX1在同一分支,属于第4亚群。In order to realize the molecular biological characteristics and variation rule of porcine reproductive and respiratory syndrome virus （PRRSV） strains in some provinces of China during 2010--2011,15 strains of PRRSV were isolated with Marc-145 cells. 15 PRRSV ORF5 genes were amplified by RT-PCR and sequences were analysized. A pair of primers were designed according to the standard sequence down- loaded in NCBI,and the amplify ORF5 gene full-length（603 bp）was amplified. The result showed that the nucleotide homology of 15 isolates is 98. 8^-99. 9~, the amino acid homology derived by nucleotide is 95.5%-99.5%. The homology is high between 15 PRRSV isolates and American type PRRSV, so the 15 PRRSV isolates are American type PRRSV. The ORF5 （151） amino acid sites of 9 PRRSV isolates in 15 PRRSV isolates have the same variation with HB-1 ,occured in R151 - K151. And the rest of 15 PRRSV isolates have the same 0RF5 （151）amino acid sites with JAX1, the ORF5 （151）is R. Phylogenetic tree showed that BFLN3-2010,BFSD1-2011,BFAH2-2011 and CH-la are in the same branch belonging to subset 2,and the rest 12 isolates and JAX1 are in the same branch belonged to subset 4.

关 键 词：猪繁殖与呼吸综合征病毒 ORF5基因 病毒分离 遗传变异 

分 类 号：S852.659.6[农业科学—基础兽医学]