干旱胁迫下黄花苜蓿与蒺藜苜蓿两个抑制性差减杂交文库的构建及分析  

Construction and analyses of two suppression subtractive hybridization libraries of Medicago falcata and Medicago truncatula under drought stress

作　　者：王天佐[1] 赵敏桂[1] 张文浩[1] 

机构地区：[1]中国科学院植物研究所植被与环境变化国家重点实验室,北京100093

出　　处：《草业学报》2012年第6期175-181,共7页Acta Prataculturae Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2007CB106800)资助

摘　　要：水分胁迫是植物面临的最主要的非生物胁迫之一,研究植物的抗旱机理以提高植物的抗旱能力具有重大的意义。抑制性差减杂交是一种筛选特异表达基因的良好手段,使用该方法成功构建了豆科植物黄花苜蓿和蒺藜苜蓿2个差减文库并进行测序。通过GO分类和KEGG分析等手段对文库中序列进行分类,并分析表达序列的差异,发现黄花苜蓿文库中对抵抗水分胁迫起积极作用的基因表达序列明显多于蒺藜苜蓿。此外,还对筛选到的2个基因在2种苜蓿中的干旱响应表达模式进行了分析,结果表明,在黄花苜蓿中这2个基因的表达更加有利于响应和抵抗干旱。这些结果在分子层面上一定程度的解释了黄花苜蓿较蒺藜苜蓿更加抗旱的原因。Drought is one of the commonest abiotic stresses for plant growth and development.Understanding the mechanisms underlying the tolerance of plants to drought stress is of critical importance for the improvement of plant drought tolerance.Suppression subtractive hybridization（SSH） is a useful tool to identify genes that are differentially expressed.In this study,two cDNA libraries from the leguminous plants Medicago falcata and M.truncatula were constructed and sequenced.The sequences of the two cDNA libraries were classified by GO and KEGG,and the differential expression was analyzed.The number of genes associated with drought resistance was greater in M.falcata than in M.truncatula.The expression levels of the two genes were positively correlated with the tolerance of the two species to drought stress and were higher in M.falcata than in M.truncatula.These results may provide a molecular explanation for some of the greater tolerance to drought stress of M.falcata than M.truncatula.

关 键 词：黄花苜蓿 蒺藜苜蓿 干旱 抑制性差减杂交(SSH) 

分 类 号：S541.9[农业科学—作物学]