海滨雀稗体细胞突变体SP2008-3的特异性分析  

Comparison and analysis of the characteristics of mutant SP2008-3 and adalayd in seashore Paspalum

作　　者：常盼盼[1,2] 钟小仙[1] 刘智微[1] 

机构地区：[1]江苏省农业科学院畜牧研究所,江苏南京210014 [2]南京农业大学动物科技学院草业科学系,江苏南京210095

出　　处：《草业学报》2012年第6期207-212,共6页Acta Prataculturae Sinica

基　　金：宁波市科技计划项目(2008C10033);慈溪市农业社会发展科技计划(CR200723)资助

摘　　要：以海滨雀稗体细胞突变体SP2008-3和Adalayd(对照)为材料,对其植物学特征和叶片表皮气孔特性进行了比较,结合染色体鉴定和RAPD技术,探索了海滨雀稗体细胞突变体SP2008-3的特异性。结果表明,与对照Ada-layd相比,SP2008-3匍匐茎节间长、茎直径分别显著减小34.77%和11.76%,叶片长度、宽度分别显著增加22.55%和11.11%,而直立茎节间长、茎直径无显著差异;叶片上表皮气孔器密度显著增加20.58%,气孔器长度和宽度分别显著减小9.64%和6.31%;叶片下表皮气孔器密度显著增加40.62%,气孔器长度和宽度分别显著减小6.73%和5.33%。SP2008-3体细胞染色体数目为2n=20,与对照Adalayd染色体数目相同。RAPD结果显示,采用随机引物S369扩增,SP2008-3缺失1条约1 100bp的条带,表明其在DNA水平上与Adalayd存在差异。A comparison of botanical properties,of stomatal characteristics of the upper and lower leaf epidermis,and of chromosome identification and RAPD analysis between mutant SP2008-3 and Adalayd in seashore Paspalum was carried out.Stolon internode length and stem diameter were reduced by 34.77% and 11.76% in SP2008-3 compared with Adalayd and leaf length and width were increased by 22.55% and 11.11% respectively.There were no significant differences in erect stem internode length and diameter between SP2008-3 and Adalayd.In the upper leaf epidermis of SP2008-3,stomatal density was 20.58% greater,and stomatal length and width were 9.64% and 6.31% less respectivley than in Adalayd.Stomatal density in the lower leaf epidermis of SP2008-3,was 40.62% greater,and stomatal length and width were 6.73% and 5.33% less than in Adalayd.The somatic chromosome number was 2n=20 in both SP2008-3 and Adalayd.RAPD analysis showed a difference in the DNA： a 1 100 bp band was missing in SP2008-3 when the DNA was amplified by primer S369.

关 键 词：海滨雀稗 体细胞 突变体 RAPD 气孔特性 植物学特性 

分 类 号：S688.4[农业科学—观赏园艺]