Nrf2基因敲除小鼠的饲养繁殖及基因型鉴定  

Breeding,Reproducing,and Identifying Nrf2 Knockout Mice

作　　者：胡春萍[1] 蔡雪婷[1] 杨洋[1] 卢悟广[1] 杨杰[1] 叶娟[1] 曹鹏[1] 

机构地区：[1]江苏省中医药研究院细胞与分子生物学实验室,江苏南京210028

出　　处：《中南医学科学杂志》2012年第6期613-615,623,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金资助项目(81274150;81201967;30873410);江苏省中医药领军人才项目(RC1006)

摘　　要：目的繁殖和鉴定Nrf2基因敲除小鼠。方法将引进的杂合子小鼠进行饲养并繁殖,繁殖成功的子代有3种基因型小鼠,即野生型、杂合子以及纯合子小鼠,从繁殖成功的小鼠尾巴中提取基因组DNA,用PCR法鉴定小鼠的基因型。结果 Nrf2杂合子小鼠的繁殖和饲养均获得成功,亦获得了较多的基因敲除纯合子小鼠。结论正确的饲养繁殖以及鉴定方法是从杂合子中获得Nrf2基因敲除纯合子小鼠的有效途径。Objective To breed and identify Nrf2 knockout mice. Methods Nrf2 knockout mice were bred and repro duced according to Mendel law. Genomic DNA was extracted from the mice tails and subjected to PCR for genotype identification. Results The experimental model for Nrf2 knockout mice was successfully established and more Nrf2 knockout mice were repro duced. Conclusion The polymerase chain reaction experiment can succeed in distinguishing different genetic Nrf2 gene of phenotype. Furthermore,it can lay out a solid foundation to further study of Nff2 knockout mice related model.

关 键 词：NRF2 基因敲除 小鼠 聚合酶链反应 

分 类 号：Q78[生物学—分子生物学]