RANTES基因多态性与乙型肝炎慢性化的相关性研究  

Correlation of single nucleotide polymorphism of chemokine RANTES to the chronicity of hepatitis B viral infection

作　　者：高宝霞[1] 高华[2] 张国顺[3] 冯福民[4] 

机构地区：[1]河北联合大学附属开滦医院感染性疾病科,河北省唐山市063000 [2]唐山市人民医院检验科 [3]河北联合大学附属医院消化内科,河北省唐山市063000 [4]河北联合大学公共卫生学院,河北省唐山市063000

出　　处：《实用肝脏病杂志》2012年第6期503-505,共3页Journal of Practical Hepatology

基　　金：河北省卫生厅医学科学研究重点课题(20100128);唐山市科技局科学技术研究与发展指导计划项目(121302019a)

摘　　要：目的探讨正常T细胞表达和分泌活性因子(RANTES)基因启动子区单核苷酸多态性与乙型肝炎慢性化的关系。方法采用聚合酶链反应-限制性片断长度多态性方法检测118例慢性乙型肝炎和61例急性乙型肝炎患者外周血RANTES基因启动子区-403、-28和In1.1三个基因位点基因分型。结果 CHB患者In1.1TC基因型(34.7%)及C等位基因频率(37.7%)明显高于对照组(21.3%,25.4%),差异有统计学意义(P﹤0.05);TC基因型慢性化的风险是TT基因型的2.2倍(OR=2.278,95%CI:1.079～4.808);多元Logistic回归分析显示TC基因型携带者与TT相比患慢性乙型肝炎的危险性增大。结论 RANTES In1.1基因位点多态性与乙型肝炎慢性化有关,携带In1.1TC基因型的HBV感染者易发生慢性化。Objective To investigate the correlation of single nucleotide polymorphisms of RANTES to the chronicity of HBV infection. Methods The restriction fragment length polymorphism-polymerase chain reaction was used to analyze the RANTES gene -403,-28 and Inl.1 polymorphisms in 118 patients with chronic hepatitis B and 61 patients with acute hepatitis B. Results The frequencies of TC genotype and C allele of Inl.lgene in patients with chronic hepatitis B were statistically higher than that in patients with acute hepatitis B （34.7% and 37.7% vs. 21.3% and 25.4%,P〈 0.05）;The risk of chronieity of HBV infection with TC genotype was 2.2 folds than that in patients with TT genotype （OR=2.278,95%CI：1.079 to 4.808）;The multivariate logistic regression analysis showed that the TC genotype also occurred more frequently than in controls. Conclusion Polymorphism of RANTES gene Inl.1 TC is associated with chronicity of HBV infection.

关 键 词：乙型肝炎 慢性化 正常T细胞表达和分泌活性因子 基因多态性 

分 类 号：R512.62[医药卫生—内科学]