Two Retroviruses Packaged in One Cell Line can Combined Inhibit the Replication of HIV-1 in TZM-bl Cells  

作　　者：Zhipin Liang Zhiyuan Guo Xin Wang Xiaohong Kong Chang Liul 

机构地区：[1]School of Medicine, Nankai University, Tianjin 300071, China, [2]Tianjin Centers for Disease Control and Prevention, Tianjin 300171, China

出　　处：《Virologica Sinica》2012年第6期339-344,共6页中国病毒学（英文版）

基　　金：National Natural Science Foundation of China(81101245,30970162);The Fundamental Research Funds for the Central Universities(65011871);National Training Programs of Innovation for Undergraduates(111005505)

摘　　要：The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1. However, the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin. In this study, two retroviral vector plasmids were constructed. One inhibited the vpu gene expression; the other one over-expressed the tetherin. Both retroviral vector plasmids could be packaged in the packaging cell line PT67 to obtain the corresponding retroviruses. The retroviral vector plasmids＇ functions of tetherin over-expression or vpu-RNAi were detected at the cell level. Retroviral vector plasmids were transfected to PT67 cells at different ratios from 0T3V to 3TOV, and then mixed retroviruses were harvested. The antiviral functions of mixed retroviruses were detected in HIV-1 infected TZM-bi cells. The results showed that packaged mixed retroviruses could repress the replication of HIV-1 in TZM-bl cells.The cellular protein tetherin tethers the HIV-1 viral particles on the cellular membrane to inhibit the replication of HIV-1.However,the HIV-1 accessory protein Vpu counteracts the antiviral function of tetherin.In this study,two retroviral vector plasmids were constructed.One inhibited the vpu gene expression;the other one over-expressed the tetherin.Both retroviral vector plasmids could be packaged in the packaging cell line PT67 to obtain the corresponding retroviruses.The retroviral vector plasmids' functions of tetherin over-expression or vpu-RNAi were detected at the cell level.Retroviral vector plasmids were transfected to PT67 cells at different ratios from 0T3V to 3T0V,and then mixed retroviruses were harvested.The antiviral functions of mixed retroviruses were detected in HIV-1 infected TZM-bl cells.The results showed that packaged mixed retroviruses could repress the replication of HIV-1 in TZM-bl cells.

关 键 词：HIV- 1 VPU TETHERIN Gene therapy 

分 类 号：Q782[生物学—分子生物学] Q78