大豆分枝数和叶柄夹角的相关野生片段分析  

Detection of Wild Segments Associated with Number of Branches on Main Stem and Leafstalk Angle in Soybean

作　　者：王吴彬[1] 何庆元[1] 杨红燕[1] 向仕华[1] 赵团结[1] 邢光南[1] 盖钧镒[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室,南京210095

出　　处：《中国农业科学》2012年第23期4749-4758,共10页Scientia Agricultura Sinica

基　　金：国家"973"重点基础研究发展计划项目(2009CB1184;2010CB1259;2011CB1093);国家"863"高技术研究发展计划项目(2011AA10A105);国家自然科学基金项目(31071442;30900902);农业部公益性行业科研专项(200803060);江苏省优势学科建设工程专项;国家重点实验室自主课题

摘　　要：【目的】从以栽培大豆为遗传背景的野生大豆染色体片段代换系(CSSL)群体中检测出与分枝数和叶柄夹角有关的野生片段,估计其遗传效应,为未来基因克隆和功能研究提供材料基础。【方法】利用由151个家系组成的野生大豆CSSL群体(SojaCSSLP1),通过单标记分析、区间作图、完备复合区间作图和基于混合线性模型的复合区间作图等四种定位方法,结合与轮回亲本有显著差异的染色体片段代换系间相互比对,检测与分枝数和叶柄夹角相关的野生片段。【结果】累计共检测到3个分枝数相关的野生等位变异/片段和5个叶柄夹角相关野生等位变异/片段,其中与分枝数相关的Sat_160野生片段和与叶柄夹角相关的Sat_286野生片段能分别被所有方法检测到。在这些QTL/片段中,Sat_286位点最高能解释22%的叶柄夹角表型变异;在所有检测到的位点(片段)上,来自野生大豆的等位基因均具有正向的加性效应,这与2个亲本的表型差异相吻合。【结论】所发现的3个分枝数和5个叶柄夹角野生等位变异/片段均来自未报道的QTL/片段,体现了野生大豆的特点。[ Objective ] The number of branches on main stem （NB） and leafstalk angle （LA） are important factors for plant type and therefore, closely related to yield potential in soybean. The wild soybean （Glycine soja Sieb et Zucc.） usually has the characteristics of multiple NB and wide LA, of which the genetic construction is of relevant meaning in the improvement of yield potential of soybean. The present study was aimed at detecting the wild segments related to the two traits and evaluating their genetic effects from a wild soybean chromosome segment substitution line population （CSSL） with cultivated soybean genetic background, so as to provide genetic materials for further gene cloning and its function study. [ Method] A wild soybean CSSL population with 151 lines, designated as SojaCSSLP1 was used to identify the wild alleles/segments for NB and LA by using joint comparisons of CSSLs significantly different from the recurrent parent based on QTL mapping with the methods of single marker analysis （SMA）, interval mapping （IM）, inclusive composite interval mapping （ICIM） and mixed linear composite interval mapping （MCIM）. [ Result ] Three NB and five LA wild alleles/segments were detected in this study. Of them, the wild segment of Sat_160 for NB and that of Sat_286 for LA could be detected repeatedly by all the four QTL mapping procedures. Among them, the LA QTL on the segment of sat 286 could explain up to 22% of the phenotypic variation. The eight wild alleles/segments for the two plant-typecharacteristics were all associated with positive effect, which coincided with the expectation of the wild parent. [ Conclusion] All the QTL/segments detected in the present study were newly identified in wild soybean, which reflected the characteristics of wild soybean.

关 键 词：野生大豆 染色体片段代换系 分枝数 叶柄夹角 株型 

分 类 号：S565.1[农业科学—作物学]