利用内皮祖细胞、膀胱上皮细胞和平滑肌细胞构建泌尿系组织工程片状移植物  

Construction of tissue-engineered sheet graft using endothelial progenitor cells, bladder epithelial cells and smooth muscle cells in urinary system

作　　者：孟令超[1] 廖文彪[1] 杨嗣星[1] 李永伟[1] 李欣慧[1] 宋超[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060

出　　处：《中华实验外科杂志》2012年第12期2396-2398,I0003,共4页Chinese Journal of Experimental Surgery

基　　金：国家青年自然科学基金资助项目（81001019）;中央高校基本科研业务费专项资金资助项目（201130202020006）

摘　　要：目的探讨内皮祖细胞（EPCs）在体内促进组织工程片状移植物新生血管生成的可行性。方法制作兔膀胱脱细胞基质（BAM），体外培养扩增兔膀胱上皮细胞、平滑肌细胞和EPCs，再将这3种细胞分别以1．0×10。／IJ种植到BAM上，培养7d后制作成组织工程片状移植物；实验组为上皮细胞-平滑肌细胞-EPCs。BAM片状移植物，对照组为上皮细胞一平滑肌细胞．BAM片状移植物。将复合物种植于兔皮下，8周后取标本做苏木素-伊红（HE）染色并进行免疫组织化学检测。结果成功培养兔膀胱上皮细胞、平滑肌细胞、EPCs。生长曲线显示3种细胞在BAM上生长分化良好。植入皮下实验结果显示，实验组HE染色及免疫组织化学显示移植物有明显的新生血管形成，上皮及平滑肌层再生良好。而对照组移植物出现挛缩，表皮不连续，血管化不明显。结论利用EPCs、膀胱上皮细胞、平滑肌细胞和BAM构建的组织工程片状移植物在体内生长良好，EPCs在体内能促进移植物新生血管形成。Objective To investigate the feasibility of endothelial progenitor cells （EPCs） promo- ting angiogenesis of tissue-engineered sheet graft using bladder epithelial cells and smooth muscle cells see- ded on the bladder acellular matrix （BAM） in vivo. Methods BAM of rabbits was made in vitro. Bladder epithelial cells, smooth muscle cells and EPCs of rabbits were cultured and expanded in vitro, then seeded on the BAM and cultured for 7 days for producing sheet graft. The experimental group was bladder epitheli- al cell,s-smooth muscle cells-EPCs-BAM sheet graft and the control group was bladder epithelial cells- smooth muscle cells-BAM sheet graft. Then the grafts were implanted into the back of rabbits. Specimens were obtained at 8th week postoperatively and were examined histologically. Results Bladder epithelial cells, smooth muscle cells and EPCs were cultured and expanded in vitro. At 8th week after operation, his- tologieal detection of specimens showed angiogenesis and epithelial coverage in the experimental group. The epithelial cells gradually integrated with the BAM and smooth muscle cells grew well. In the control group, the graft showed contracture and discontinuous epithelial lay, and there was no obvious vascularization. Conclusion Tissue-engineered sheet graft constructed by EPCs, bladder epithelial cells and smooth mus- cle cells grows well in vivo. EPCs can promote angiogenesis of tissue-engineered sheet graft.

关 键 词：膀胱脱细胞基质 上皮细胞 内皮祖细胞 组织工程 

分 类 号：R318.0[医药卫生—生物医学工程]