大鼠骨髓间充质干细胞包膜下移植对肾间质纤维化的作用  

Effect of subcapsular transplantation of bone marrow mesenchymal stem cells on obstruction-in- duced renal interstitial fibrosis in rats

作　　者：程庆[1] 刘振湘[1] 尹先来[1] 邓湘东[2] 曹卉[2] 王顺兰[2] 谈顺[3] 白志明[1] 

机构地区：[1]海口市人民医院泌尿外科,570208 [2]海口市人民医院中心实验室 [3]海口市人民医院病理科

出　　处：《中华实验外科杂志》2012年第12期2416-2418,共3页Chinese Journal of Experimental Surgery

基　　金：海南省自然科学基金资助项目（811151）

摘　　要：目的观察大鼠骨髓间充质干细胞（BMSCs）经肾包膜下移植对可复性单侧输尿管梗阻模型（R—UUO）大鼠。肾间质纤维化及纤维化过程中炎性反应的影响，探讨包膜下移植的安全性。方法54只4月龄SD大鼠随机分为实验组（R—UUO＋MSCs）、对照组（R．UUO＋DMEM）、损伤对照组（R-UUO），每组18只，肾包膜下注射法移植后，于3、7、14d随机于每组内取6只大鼠，取患肾。免疫组织化学检测单核／巨噬细胞表面特异性标志抗原（ED-1）表达，Masson染色检测胶原沉积量。结果（1）和对照组（0．000773±0．000380）比较，实验组（0．000384±0．000407）在第14天的ED-1表达水平显著降低（P〈0．05），但胶原沉积量未见改善。（2）与损伤对照组（0．000293±0．000281）比较，对照组（0．000773±0．000380）在第14天时ED-1表达显著增加（P〈0．05），但胶原沉积量未见有显著增加。结论MSCs包膜下移植可抑制肾间质纤维化过程中的巨噬细胞浸润，但短期内不能改善纤维化。包膜下注射短期内有明确损伤，但并未造成纤维化水平加重。Objective To investigate the efficacy and safety of mesenchymal stem cells （MSCs） subcapsular injection in R-UUO model of rats by observing the changes in macrophage infiltration and collagen deposition. Methods A total of 54 S-D rats were randomly divided into three groups （ n = 18 each） , including the MSCs delivered group, DMEM delivered group and R-UUO group. At the day 3, 7 and 14 af- ter subeapsular transplantation of MSCs or vehicle, the 4 I^m thick sections of the kidney were stained with the Masson trichrome technique. Immunohistochemical staining was performed with mouse anti-CD68 anti- body （ ED-1 ）. Results （ 1 ） The subeapsular transplantation of MSCs significantly reduced the mean 1.4 of ED-1 expression at 14th day after transplantation （ P 〈 0. 05 ）, but didn＇ t attenuate the collagen deposi- tion; （2） The subcapsular transplantation of MSCs caused a significant rise in ED-1 mean IA as compared with sham-operated group on the 14th day （ P 〈 0.05 ）, but didn＇ t augment the interstitial fibrosis. Con- dusion The subcapsular transplantation of MSCs in R-UUO rats could ameliorate the macrophage infiltra- tion in interstitium, but didn＇ t improve the interstitial fibrosis within 2 weeks. The subeapsular transplantation of MSCs aggravates inflammation of obstructive nephropathy on the 14th day after transplantation, but doesn＇t contribute to the interstitial fibrosis.

关 键 词：间充质干细胞 肾间质纤维化 移植 巨噬细胞 损伤 

分 类 号：R699.2[医药卫生—泌尿科学]