慢性荨麻疹患者红细胞补体受体1分子表达及外周血IgE和补体C3、C4水平的相关性研究  

Expression of complement receptor type 1 on erythrocytes and its correlation with immunoglobulin E, complement C3 and C4 expressions in patients with chronic urticaria

作　　者：罗颖[1] 晏洪波[1] 周凌[1] 杨李 夏罡[1] 

机构地区：[1]广州军区武汉总医院皮肤性病科,武汉430070 [2]医学实验科

出　　处：《中华皮肤科杂志》2012年第12期855-858,共4页Chinese Journal of Dermatology

摘　　要：目的探讨红细胞补体受体1（CR1）在慢性荨麻疹发病机制中的作用及意义。方法慢性荨麻疹患者59例，其中含人工划痕症14例，慢性特发性荨麻疹45例，采用流式细胞仪检测外周血红细胞CR1的表达，并采用双抗体夹心酶联免疫吸附法（EusA）检测外周血补体c3、C4、CH50及IgE水平。29例健康人作为对照组。利用ONE—WAYANOVA进行三组样本间均数比较，两组均数的比较采用独立样本t检验，相关分析采用Pearson相关分析法。结果外周血红细胞CR1的表达水平人工划痕症组为35．06±2．06（10000个红细胞表面的荧光强度）、慢性特发性荨麻疹组为29．17±1．53，均高于健康对照组（20．46±2．57），t值分别为4．20、3．33，P值均〈0．05，人工划痕症组与慢性特发性荨麻疹组间差异无统计学意义（P〉0．05）。外周血IgE水平人工划痕症组为（769．89±123．06）μg／L，慢性特发性荨麻疹组为（340．09±29．74）μg／L，均高于健康对照组（107．63±88．79肛g／L），t值分别为5．58、5．85，P值均〈0．05，人工划痕症组高于熳性特发性荨麻疹组（t=3．49，P〈0．05）。慢性荨麻疹患者中IgE为0～240μg／L的22例患者CR1水平（24．45±10．83）与IgE为500μg／L以上的17例患者CR1水平（33．09±11．86）差异有统计学意义（t=3．33，P〈0．05）。血清总IgE与CR1水平呈显著正相关（r=0．27，P〈0．05），与C3（r=0．16，P〉O．05）、C4（r=-0．08，P〉0．05）均无相关性。c3与c4具有正相关（r=0．54，P〈0．01）。3组c3、c4和CH50水平经ONE—WAYANOVA检验，差异均无统计学意义（P〉0．05）。结论红细胞CR1在慢性荨麻疹患者中的表达存在异常。Objective To investigate the role of erythrocyte complement receptor type 1 （CR1） in the pathogenesis of chronic urticaria. Methods Venous blood samples were collected from 59 patients with chronic urticaria （including 14 cases of dermatographism and 45 chronic idiopathic urticaria） and 29 healthy human controls. Flow cytometry was carried out to quantify the expression level of CR1, and double-antibody sandwich enzyme-linked immunosorbent assay to determine the serum level of immunoglobulin E （IgE）, complement C3, C4 and 50% complement hemolytic activity （CHS0）. Differences in these parameters were analyzed by one-way ANOVA and independent samples t-test, and correlation between these paramenters by Pearson correlation analysis. Results The expression level （expressed as mean fluorescence intensity per 10 000 erythrocytes） of CR1 was significantly higher in patients with dermatographism and chronic idiopathic urticaria than in the healthy controls （35.06 ± 2.06 and 29.17 ± 1.53 vs. 20.46 ± 2.57, t = 4.20 and 3.33, both P 〈 0.05）, while no statistical difference was observed between the patients with dermatographism and chronic idiopathic urticaria （P 〉 0.05）. Increased total serum IgE levels were observed in patients with dermatographism and chronic idiopathic urticaria compared with the healthy controls （（769.89 ± 123.0） μg/L and （340.09 ± 29.74） μg/L vs. （107.63 ± 88.79） μg/L, t = 5.58, 5.85, both P 〈 0.05 ）, and in patients with dermatographism compared with those with chronic idiopathic urticaria （t = 3.49, P 〈 0.05）. For patients with chronic urticaria, there was a statistical difference in the expression level of CR1 between individuals （n=22） with total serum IgE levels ranging from 0 to 240 μg/L and those （n = 17） higher than 500 μg/L（24.45 ± 10.83 vs. 33.09 ± 11.86, t =3.33, P 〈 0.05）. The total serum IgE levels were positively correlated with the level of CR1 （r = 0.27, P 〈 0.05）, but uncorrelated with that ofcompl

关 键 词：荨麻疹 受体 补体 补体C3 补体C4 免疫球蛋白E 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]