鸡传染性支气管炎病毒广西分离毒株抗原相关性的研究  

Evaluation of Antigenic Relationship of Guangxi Isolates of Infectious Bronchitis Virus

作　　者：王秀英[1] 李孟[1] 韦平[1] 陈秋英[1] 韦正吉[1] 磨美兰[1] 韦天超[1] 

机构地区：[1]广西大学养禽与禽病研究所,南宁530005

出　　处：《病毒学报》2012年第6期621-627,共7页Chinese Journal of Virology

基　　金：国家自然科学基金(31160516);广西科技攻关项目(0993009-2);南宁市科学研究与技术开发计划项目(200901028B;201001021B)

摘　　要：本研究将26个传染性支气管炎病毒(Infectious bronchitis virus,IBV)广西分离毒株以及参考株M41和常用疫苗株H120、Ma5和4/91共30个毒株,分别与根据这些毒株S1基因高变区Ⅰ的基因分型结果而选取的属于3个不同亚群的7个代表性分离毒株和常用疫苗株H120、Ma5和4/91制备的共10个单因子血清,在鸡胚气管环培养(TOC)上进行病毒中和试验,然后根据中和试验结果对1985～2008年间课题组所分离的26个IBV广西地方流行毒株与3个常用疫苗株H120、Ma5和4/91以及参考毒株M41的抗原相关性及其血清型进行分析。结果显示,30个试验的毒株分属7个不同的血清型,其中26个分离株有两个优势血清型(占总分离株的68%),分别是血清1型(包含13个毒株)和血清2型(包含5个毒株)。此外,我们还将分离毒株的血清分型结果与重要抗原基因(包括S1、N、M和3′UTR)的分型结果之间的关系进行了比较,发现它们之间的分型结果不尽相同。研究结果表明,近年来广西存在多个血清型IBV的流行而且不同时期流行的优势血清型不同,分离毒株之间以及分离毒株与疫苗毒株之间的抗原相关性也存在着差异。Monovalent antisera of 3 vaccine strains and 7 representative field isolates were prepared based on the comparison of genetic diversity of the hypervariable regionⅠof S1 gene（HVRⅠfrom 3 infectious bronchitis（IB） vaccine strains（H120,Ma5 and 4/91）,one reference strain M41 and 26 IB field isolates.These 30 strains were classified in 7 different genotypes,respectively.Virus-neutralizing test on tracheal organ cultures（TOC） with chicken embryo were used to evaluate relatedness values of the antigenicity based on the antibody titer,to analyze the antigenic relationships between the isolates and vaccine strains,as well as to determine the serotypes of 26 IB viruses isolated from the field in Guangxi between 1985 and 2008.The results showed 30 strains were classified into 7 distinct serotypes and there were two predominant serotypes within the 26 isolates,serotypes 1（totally 13 isolates） and serotype 2（totally 5 isolates）,respectively.In addition,there were some differences observed between the results of serotyping and the genotyping（including the S1,N,M and 3′UTR）.The results of the study demonstrated that there were different predominant serotypes and multiple serotypes of IBV circulated in Guangxi in recent years,antigenic variation existed between Guangxi field isolates and vaccine strains.

关 键 词：鸡传染性支气管炎病毒 气管环培养 病毒中和试验 中和滴度 血清型 基因型 抗原变异 

分 类 号：S852.65[农业科学—基础兽医学]