两种单负链RNA呼吸道病毒——流感病毒和呼吸道合胞病毒感染对粘蛋白1表达调控差异的研究  

Differences of the Regulation on the Expression of Mucin 1 Induced by Two Single-Strand RNA Viruses,Respiratory Syncytial Virus and Influenza Virus

作　　者：卢昕[1] 倪淑媛[2] 李煜生[3] 

机构地区：[1]南方医科大学第一临床医学院,广州510515 [2]广州医学院第三附属医院,广州510150 [3]南方医科大学基础医学院病理生理教研室,广东省蛋白质组学重点实验室和教育部重大疾病的转录组和蛋白质组学重点实验室,广州510515

出　　处：《病毒学报》2012年第6期639-644,共6页Chinese Journal of Virology

基　　金：国家自然科学基金青年基金(No.81100008);广东省高校优秀青年创新人才培育项目(No.LYM10048)

摘　　要：为进一步了解流感病毒(IFZ)是否像呼吸道合胞病毒(RSV)一样在感染过程中可以上调呼吸道上皮细胞粘蛋白1(MUC1)的表达,进而限制炎症的发展。我们利用qRT-PCR和Western Blot检测两种呼吸道单负链RNA病毒感染人呼吸道上皮细胞系——A549细胞后对MUC1的表达调控。分别利用人喉上皮细胞系——HEp-2细胞系和MDCK细胞系培养并收集RSV和IFZ。相同滴度的两种病毒分别感染A549细胞24h后,分别裂解各组细胞,收集总RNA,应用qRT-PCR检测MUC1mRNA的表达情况;或相同滴度的两种病毒分别感染A549细胞24h和48h后,裂解细胞收集总蛋白,同时收集细胞培养上清,应用Western Blot检测MUC1蛋白的表达情况,应用ELISA检测上清中TNF-α水平。结果显示,虽然两种病毒都能上调TNF-α水平,但只有RSV可以上调呼吸道上皮细胞MUC1表达,并呈剂量效应,而IFZ不能上调呼吸道上皮细胞MUC1的表达。本研究首次探讨两种常见呼吸道单负链RNA病毒感染呼吸道上皮细胞后对MUC1表达调控的差异,初步证实临床上IFZ感染后病情自限性的机制不同于RSV感染,与MUC1的表达上调无关。To investigate whether influenza virus（IFZ） could up-regulate the expression of mucin 1（MUC1） which exists in epithelial cells of upper respiratory track to restrict the inflammation,as respiratory syncytial virus（RSV） does.Quantitative RT-PCR and Western Blot were performed to detect the expression level of MUC1 induced by two single-strand RNA viruses in A549 cell lines.HEp-2 and MDCK cells were used respectively to culture RSV and IFZ.At 24h post A549 cells infection with the same titer of RSV or IFZ,the total RNA was harvest,qRT-PCR was then performed to observe the expression level of MUC1 mRNA.Meanwhile,at 24 h and 48 h post A549 cells infection with the same titer of RSV or IFZ,the total protein and supernatant were collected respectively after cell lysis,Western Blot was then used to detect the expression level of MUC1.Results showed that RSV could up-regulate the expression of MUC1 in airway epithelial cells with a significant dose-effect correlation,whereas IFZ could not.This study firstly investigated the differences of the regulation on the expression of MUC1 induced by two single-strand RNA viruses,and demonstrated initially that the mechanism of IFZ self-limiting differed from RSV,which attributed to up-regulation of the expression level of MUC1.

关 键 词：流感病毒 呼吸道合胞病毒 呼吸道上皮细胞 粘蛋白1 炎症调控 

分 类 号：R373.1[医药卫生—病原生物学]