房水和血清中单核细胞趋化蛋白-1和巨噬细胞游走抑制因子变化与2型糖尿病视网膜病变的关系  

The relationship of aqueous and serum monocyte chemotactic protein-1 and macrophage migration inhibitory factor level with diabetic retinopathy in type 2 diabetic patients

作　　者：褚利群[1] 董宁[1] 肖林[1] 徐冰[1] 刘晶[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院眼科,北京100038

出　　处：《中华实验眼科杂志》2012年第12期1122-1126,共5页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景研究发现，巨噬细胞和白细胞等炎性细胞参与糖尿病视网膜病变（DR）的发生发展，有多种细胞因子在DR的发生发展过程中发挥促进作用，但DR患者的房水及血清中单核细胞趋化蛋白-1（MCP-1）和巨噬细胞游走抑制因子（MIF）水平的变化与DR病程的关系尚不完全清楚。目的探讨2型糖尿病患者房水中MCP-1和MIF的水平与DR病程的关系。方法纳入2010年9月至2011年6月在北京世纪坛医院眼科和内分泌科确诊的2型糖尿病合并白内障并已行白内障超声乳化手术或超声乳化联合玻璃体切割术患者80例，根据眼底情况分为无DR（NDR）组20例、非增生型DR（NPDR）组38例和增生型DR（PDR）组22例，并收集同期的非糖尿病白内障手术患者即对照组26例，各组患者年龄、性别分布的差异均无统计学意义。所有患者于术中抽取未稀释的房水0．1ml，分别采用ELISA法检测房水中MCP-1和MIF的质量浓度并进行组间比较。结果PDR组、NPDR组、NDR组、对照组房水中平均MCP-1的质量浓度分别为（1660．78±562．98）、（1463．26±623．41）、（686．76±186．16）、（494．35±148．59）ng／L，总体比较差异有统计学意义（F=37．968，P=0．000），对照组与NDR组以及NPDR组与PDR组房水中MCP-1的质量浓度比较差异均无统计学意义（P=0．169、0．117）；NDR组以及NPDR组与PDR组房水中MCP-1的质量浓度均明显高于对照组，差异均有统计学意义（P=0．000）。PDR组、NPDR组、NDR组、对照组患者房水中平均MIF的质量浓度分别为（6．85±1．99）、（3．56±0．90）、（1．10±0．48）、（0．86±0．46）μg／L，总体比较差异有统计学意义（F=144．502，P=0．000）；对照组与NDR组房水中MIF的质量浓度比较差异无统计学意义（P=0．475）；其余各组间两两比较差异均有统计学意义（P=0．000）。所有受检者房水中MCP-1与MIF质量浓度变化呈正相关Background Various studies have suggested that inflammatory factors such as leucocytes and macrophages are involved in the occurrence and development of diabetic retinopathy （DR） , and many cytokines promote the occurrence of DR. However,the relationship of aqueous and serum monocyte chemotactic protein-1 （MCP-1） and macrophage migration inhibitory factor （MIF） change with DR is unclear. Objective This study was to investigate the effects of MCP-1 and MIF in aqueous and serum during DR development. Methods Eighty patients with type 2 diabetes were enrolled from Beijing Shijitan Hospital. These patients received phacoemulsification or phacoemulsification and vitrectomy from September,2010 to June,201 I. Twenty-six cataract patients in the same stage （without diabetes） who underwent phacoemulsification surgery served as controls. According to the clinical stage of the DR,the diabetic patients were classified as the non-DR group （NDR） （20 eyes）, non-proliferative DR group （NPDR） （38 eyes） and proliferative DR group （PDR） （22 eyes）. Aqueous humour and periphery blood samples were collected during the operation to detect MCP-1 and MIF using enzyme-linked immunosorbent assay （ELISA）. Written informed consent was obtained from each subject before any relevant medical examination. Results The average aqueous MCP-1 levels were（ 1660. 78_＋562.98）, （ 1463.26_＋ 623.41 ）, （686.76＋186. 16） and （494. 35＋148.59） ng/L in the PDR group, NPDR group, NDR group and control group, respectively, showing a significant difference among the 4 groups （F= 37. 968, P= 0. 000）. No significant differences were found in the aqueous MCP-1 levels between the control group and NDR group （P=0. 169） ,or between the NPDR group and PDR group （P=0. 117）. However,the aqueous MCP-1 levels were significantly elevated in the PDR group,NPDR group and NDR group compared with the control group （P=0. 000）. The average aqueous MIF levels were （6.85_＋1.99）, （3.56-＋0.90�

关 键 词：糖尿病视网膜病变 单核细胞趋化蛋白-1 巨噬细胞游走抑制因子 房水 

分 类 号：R587.2[医药卫生—内分泌]