不同辐射剂量损伤后T淋巴细胞亚群及Th1和Th2的变化  

Changes of T-lymphocyte subsets, Th1 and Th2 after different doses of irradiation

作　　者：涂序珉[1] 郑辉[1] 甄荣[1] 张志兴[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《癌变．畸变．突变》2012年第6期410-412,417,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:观察不同辐射剂量损伤对小鼠T淋巴细胞功能亚群的影响,探讨辐射所致的免疫系统损伤在细胞学和分子水平60上的机制。方法:C57BL/6j小鼠采用Coγ射线照射诱导辐射损伤模型。总照射剂量分别为0、0.7、1.4、2.8和5.6Gy。应用细胞表面标志和细胞内因子标记,流式细胞仪检测分析不同剂量组小鼠脾T淋巴细胞功能亚群CD3+CD4+CD8+T细胞改变及Th1和低幅度与受照剂量明显相关。CD+4/CD8+比值显示照射后均明显升高(P<0.01)。照射后Th1水平均降低(P<0.01),而在照射剂量≥1.4Gy时Th2均明显降低(P<0.01),其中Th1降低明显高于Th2。受照剂量大于2.8Gy组Th2/Th1比值较空白对照组明显升高(P<0.01)。结论:不同照射剂量对T淋巴细胞功能亚群CD3+、CD4+、CD8+T细胞和CD4+/CD8+比值有明显影响,Th1,Th2和Th2/Th1功能亚群的失衡在辐射所致的免疫损伤中具有重要作用。OBJECTIVE：To observe the effects on T-lymphocyte subsets after different doses of irradiation. To investigate the cellular and molecular mechanisms of immune system injury induced by irradiation. METHODS：The C57BL/6j mice were divided into normal control group and model groups. The mice in radiation injury model were exposed to 60Co gamma rays （0.7, 1.4, 2.8 and 5.6 Gy）. The surface markers and intracellular cytokines of lymphocytes were stained with fluorescence labeled monoclonal antibodies . The changes of T-lymphocyte subsets CD3＋,CD4＋,CD8＋, IFN-r and interleukin-4 in spleen celles were analyzed by flow cytometry. RESULTS：T-lymphocyte subsets CD3＋, CD4＋,CD8＋ were distinctly reduced after irradiation. This change was related to gamma ray doses. The CD4＋/CD8＋ was significantly increased. The Th1 and Th2 were depressed after irradiation and the decrement level of Th1 was larger than Th2. When irradiation dose was over 2.8 Gy, Th2/Th1 showed a marked increase compared with control group. CONCLUSION：Changes of mice T-lymphocyte subsets CD3＋,CD4＋,CD8＋ and CD4＋/CD8＋ ratio could be caused by different doses of irradiation, especially the imbalance of Th1, Th2 and Th2/Th1 ratio that played an important role in radiation-induced immune injury.

关 键 词：辐射免疫损伤 T淋巴细胞功能亚群 TH1 TH2 

分 类 号：Q691[生物学—生物物理学]