^(131)I-rFliC的制备及乳腺癌荷瘤鼠体内生物学分布特征  

Preparation of ^(131)I-rFliC and its biodistribution in breast cancer-bearing mice

作　　者：杨欢[1] 梁婷[1] 张超[1] 宋静[1] 郝静[1] 侯桂华[1] 

机构地区：[1]山东大学医学院实验核医学研究所,济南250012

出　　处：《山东大学学报（医学版）》2012年第12期31-36,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81071172);山东省自然科学基金(ZR2010CM025)

摘　　要：目的探讨放射性碘131(131I)标记基因重组鞭毛蛋白(rFliC)在乳腺癌荷瘤小鼠体内的生物分布特征及意义。方法逆转录聚合酶链反应(RT-PCR)、蛋白质印迹法(Western blot)及免疫荧光检测人乳腺腺癌细胞系(MCF-7)中rFliC的受体TLR5的表达;四氯二苯基苷脲(Iodogen)法131I标记rFliC,检测其放射化学纯度、稳定性、细胞摄取状况;制备MCF-7荷瘤鼠,注射131I-rFliC后,观察其生物学分布及肿瘤靶向性。结果①MCF-7表达TLR5的mRNA及蛋白;随着rFliC浓度的增加,TLR5 mRNA及蛋白表达逐渐减弱;②131I-rFliC标记率达95.19%;放射化学纯度为96.46%;在血清中稳定性高,可被MCF-7稳定摄取;③131I-iFliC主要经肝肾代谢,肿瘤靶向性明显,24 h靶/肌肉(T/M)放射性比值为7.09,可获得清晰放射自显影像。结论131I-rFliC肿瘤靶向明显,有望作为一种新型分子探针监测乳腺癌的发生发展。Objective To investigate the biodistribution and significance of 131I- rFliC in breast cancer-bearing mice. Methods （1） The expression of TLR5 was assayed by the immunofluorescence method,RT-PCR and Western blot. （2） rFliC was labeled by 131I and the radiochemical purity, stability, cell uptake and efflux of 131 I- rFliC were identified. （3） MCF-7 bearing mice were prepared and injected with 131 I- rFliC. The biodistribution of 131 I- rFliC and tumor targeting were analyzed. Results （1） MCF-7 could express TLR5 at the mRNA and protein levels. The expression of TLR5 was down-regulated by rFliC with a dose-dependem manner. （2） The labeling efficiency of 131I-rFliC was 95. 19% and the radiochemical purity was 96.46%. 131I-rFliC was more stable in serum and could be absorbed by MCF-7 stably. （3） 24 h after injection with 131I-rFliC, T/M ratio was 7.09, and tumor tissue appeared clearly with the autoradiography. Con- clusion Tumor-targeting of 131I -rFliC is obvious, and it may become a new molecular probe for the diagnosis and therapy of breast cancer.

关 键 词：鞭毛蛋白 TOLL样受体5 放射性碘标记 体内分布 乳腺肿瘤 

分 类 号：R817.3[医药卫生—影像医学与核医学]