骨髓间充质干细胞对仓鼠皮肤深Ⅱ度烫伤的治疗作用及机制研究  

Effect of Bone Marrow Mesenchymal Stem on Deep Partial Thickness Derma Burn Injury in Hamsters

作　　者：马嵋[1,2] 张燕[2] 魏琴[1] 姜涛[1] 段明军[1] 张春[1] 王雪梅一 侯月梅[1] 

机构地区：[1]新疆医科大学第一附属医院新疆医学动物模型研究重点实验室,乌鲁木齐830054 [2]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《实验动物与比较医学》2012年第6期499-502,共4页Laboratory Animal and Comparative Medicine

基　　金：新疆医科大学第一附属医院干细胞专项基金（2010GXB03）

摘　　要：目的观察骨髓间充质干细胞（BMMSCs）移植对仓鼠皮肤深Ⅱ度烫伤的治疗作用以及对其机制研究。方法将40只仓鼠复制皮肤深Ⅱ度烫伤模型并随机分为：干细胞移植组（A组）和模型对照组（B组），将体外培养至第5代的同种异体BMMSCs，数目为1×107／ml，通过局部注射加表面涂抹上清的方法移植至干细胞移植组烫伤部位，对照组给予等量的生理盐水，观察愈合效果，分别于移植后1、3、7、14d每组5只取材行HE染色进行病理学观察，PCR检测皮肤肥大细胞糜蛋白酶数量。结果千细胞移植组愈合速度快于对照组，HE染色结果显示移植组愈合质量好于对照组，PCR检测结果显示，两组均检测到糜蛋白酶表达，在第3日表达最高。结论BMMSCs能促进创面愈合，皮肤肥大细胞糜蛋白酶可能参与了创面愈合过程。Objective To investigate the effect of bone marrow mesencymals stem cells transplan- tation on the deep partial thickness derma burn injury in hamsters and explore the possible mechanism. Methods Fourty hamsters were used to create deep partial thickness derma burn injury model and randomly divided into two groups： transplantation group （group A） and control group （group B）. The wound of animals in group A were treated with local transplantation of the fifth generation cells of MSCs cultured in vitro with concentration of 1×107/ml plus surface daub of supernatant. The group B were treated with the same amount of saline. The wound healing effect was observed by HE staining, chymase mRNA level was analyzed on day 1, 3, 7, 14 after transplantation. Results The HE staining results showed the quality of healing was better in transplantation group than in control group. PCR results showed that chymase gene expression was found in both groups with the highest expression on day 3. Conclusion BMMSCs transplantations can promote wound healing, and dermal mastocyte chymotrysin would be involved in wound healing process.

关 键 词：骨髓间充质干细胞 烫伤 仓鼠 糜蛋白酶 

分 类 号：R644[医药卫生—外科学] R-332[医药卫生—临床医学]