红外偏振光对骨髓间充质干细胞的影响  

The effect of linear polarized near-infrared on bone marrow stromal stem cells

作　　者：毕迎立[1] 范建中[1] 陈银海[2] 

机构地区：[1]南方医科大学南方医院康复医学科,广州510515 [2]南方医科大学珠江医院门诊部

出　　处：《中国康复医学杂志》2012年第12期1093-1096,共4页Chinese Journal of Rehabilitation Medicine

基　　金：广东省自然科学基金项目(8451051501000460);广州市海珠区科技计划项目(2009-Y-018)

摘　　要：目的:观察红外偏振光治疗仪的临床治疗剂量对骨髓间充质干细胞(BMSCs)生长情况的影响。方法:采用全骨髓培养法获取SD大鼠的原代BMSCs,取传代培养的细胞分为:实验组:红外偏振光处理10min;对照组:空气暴露相同时间。采用CCK8法检测细胞的增殖活性,流式细胞术进行细胞凋亡检测和细胞周期分析。结果:CCK8法检测结果显示,实验组的光密度(OD)值与对照组相比,差异无显著性意义(P>0.05);细胞凋亡检测显示,两组的结果差异无显著性意义(P>0.05);细胞周期分析结果显示,两组差异无显著性意义(P>0.05)。结论:红外偏振光的临床治疗剂量对骨髓间充质干细胞的增殖无明显影响,没有发现引起细胞凋亡,对细胞周期无明显影响。Objective： To investigate the effect of linear polarized near-infrared on the growth of bone marrow stromal stem cells （BMSCs）. Method： The primary BMSCs were got from Sprague-Dawley rats with the method of whole bone marrow adher- ent cultivation. The passage cells were divided into trial group with linear polarized near-infrared irradiation for 10min and control group with disposing for the same time without linear polarized near-infrared irradiation. The condition of cell proliferation was measured using CCK8 method. The cell apoptosis and cell cycle were analysed with flow cytometry （FACS）.Result： CCK8 assay revealed no significant change of optical density（OD） in proliferation of the cells irradiated by linear polarized near-infrared（P 〉 0.05）. Flow cytometry results had no significant variation of cell apoptosis （P 〉 0.05）, nor had significant variation of cell cycle （P 〉 0.05）. Conclusion： The linear polarized near-infrared with clinical treatment dosage has no effect on the proliferation, apoptosis and cell cycle of BMSCs.

关 键 词：红外偏振光 骨髓间充质干细胞 增殖 凋亡 细胞周期 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] R454.2[医药卫生—基础医学]