电针对去卵巢骨质疏松大鼠骨代谢生化指标及肿瘤坏死因子-α和骨胶原代谢生化指标的影响  

Effect of electroacupuncture on the biochemical indices of bone and bone collagen metabolism and TNF-α in osteoporosis model rats without ovaries

作　　者：鲍圣涌 张少君 林婉娟 陈竞芬 

机构地区：[1]暨南大学第二临床医学院附属深圳市人民医院理疗科,广东深圳518020

出　　处：《中国针灸》2012年第12期1108-1112,共5页Chinese Acupuncture & Moxibustion

基　　金：深圳市科技计划资助项目:医疗卫生类200802091

摘　　要：目的:探讨电针治疗绝经后骨质疏松症的可能作用机制。方法:采用60只6月龄雌性SD大鼠,切除双侧卵巢后饲养90d,造成实验性骨质疏松症动物模型,然后随机分成模型对照组(不做任何针刺治疗)、常规取穴组(针刺"脾俞""胃俞""肾俞""气海俞")、补益电针组(针刺"关元""足三里"穴)、通瘀电针组(针刺"肾俞""膈俞""大杼"穴)、补益通瘀组(针刺"关元""足三里""肾俞""膈俞""大杼"穴)5组,每组12只,电针治疗每天1次,每次30min,连续治疗6d后休息1d,共治疗12周。采用酶联免疫法检测肿瘤坏死因子(TNF-α),放射免疫法测定雌二醇(E2)、骨钙素(BGP)及Ⅰ型前胶原羧基末端前肽(PICP)、Ⅰ型胶原交联羧基末端肽(ICTP),对硝基苯酚磷酸盐法测定碱性磷酸酶(ALP),全自动生化分析仪测定尿钙(Ca)、磷(P)、脱氧吡啶啉(DPD)、肌酐(Cr)。结果:与模型对照组比较,所有电针组尿DPD/Cr、Ca/Cr、血清ALP、BGP及TNF-α、ICTP值均显著降低(均P<0.01),血清E2、PICP值均显著升高(均P<0.01);与补益通瘀组比较,另外3个电针组的尿DPD/Cr、Ca/Cr比较差异无统计学意义(均P>0.05),血清ALP、BGP及TNF-α、ICTP值均显著升高(均P<0.01),血清E2、PICP值均显著降低(均P<0.01)。结论:电针能够使绝经后骨质疏松症模型大鼠尿DPD/Cr、Ca/Cr、血清ALP、BGP及TNF-α、ICTP值显著降低,血清E2、PICP含量显著升高,且"补益通瘀电针法"效果明显优于其他针刺方法。Objective To explore the mechanisms of electroacupuncture （EA） on postmenopausal osteoporosis （PMO）. Methads Sixty female SD rats aged 6 months were selected, reseeted double ovaries and fed for 90 days in order to make the model of experimental osteoporosis, and then, they were randomly divided into a model control group （without any therapy）, a routine acupoints applying group, which were treated with EA at ＂Pishu＂ （BL 20）, ＂Weishu＂ （BL 21）, ＂Shenshu＂ （BL 23） and ＂Qihaishu＂ （BL 24）. and an EA with tonification method （EAT） group at ＂Guanyuan＂ （CV 4） and ＂Zusanli＂ （ST 36）, and an EA with dispersing blood stasis method （EAD） group at ＂Shenshu＂ （BL 23）, ＂Geshu＂ （BL 17） and ＂Dazhu＂ （BL 11）, and an EA with tonification and dispersing blood stasis method （EATD） group at ＂Guanyuan＂ （CV 4）, ＂Zusanli＂ （ST 36）, ＂Shenshu＂ （BL 23）, ＂Geshu＂ （BL 17） and ＂Dazhu＂ （BL 11）, 12 rats in each group. EA therapy was performed once a day, 30 rain each time. Then these rats rested for 1 day after consecutive treatment for 6 days. The treatment lasted for 12 weeks. Enzyme linked immunosorbent assay （ELISA） was used to examine the level of TNF-a, and the level of Ez, osteo calin （BGP） and earboxyterminal propeptide of type I procollagen （PICP）, carboxyterminal telopeptide of type I lollagen （ICTP） by radioimmunoassay, and alkaline phosphatase by p-nitrophenylphosphate method, and Ca, P, Deoxypyridinoline （DPD） and creatinine （Cr） in urine were detected by automatic biochemistry analy- zer. Results Compared with model control group, the level of DPD/Cr, Ca/Cr, and the serum contents of ALP, BGP, TNF-a and ICTP in all EA groups decreased significantly （all P-（0.01）, the level of Ezand PICP in serum increased significantly （all P,（0.01）. Compared with EATD group, the level of DPD Cr and Ca/Cr in the otherthree EA groups had no statistical differences Call P〉0.05）, but

关 键 词：去卵巢大鼠 骨质疏松 绝经后 电针 肿瘤坏死因子-Α PICP ICTP 

分 类 号：R246.3[医药卫生—针灸推拿学]