某地区3～6周岁健康儿童干湿化学分析仪6个血清酶项目参考区间的建立与比较研究  

Establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in a region

作　　者：曹科[1,2] 马东礼[1] 罗小娟[1] 黄宝兴[1] 吴跃平[1] 马金香[3] 

机构地区：[1]广东省深圳市儿童医院检验科,518026 [2]广州医学院研究生课程进修班,广州510182 [3]广州医学院公共卫生与全科医学学院预防医学系,广州510182

出　　处：《重庆医学》2012年第34期3629-3631,共3页Chongqing medicine

摘　　要：目的建立和比较深圳地区3～6周岁健康儿童干湿化学分析仪6个血清酶项目的参考区间,更好地为临床诊断和治疗服务。方法采用随机分层抽样筛选出391名健康儿童抽取静脉血,用美国强生Vitros 350干式化学分析仪和美国Beck-man Coulter LX20湿化学分析仪进行血清天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰转肽酶(GGT)、肌酸激酶(CK)检测并分析结果。结果该地区3～6周岁健康儿童两种方法内不同性别血清AST、LDH、ALT、ALP、GGT结果比较,差异均无统计学意义(P>0.05);CK结果差异均有统计学意义(P<0.05);6个血清酶项目两种方法间结果比较,差异均有统计学意义(P<0.05)。结论 6个血清酶项目两种方法间结果比较均有显著性差异,应该针对不同方法学分别建立该地区3～6周岁健康儿童干湿化学分析仪的参考区间。Objective To establish and compare the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in Shenzhen,provide better references for clinical diagnosis and treatment. Methods The stratified randomized cluster sampling method was used to select 391 healthy children aged from 3 to 6 years,AST. LDH. ALT. ALP.GGT.CK in venous blood were detected by the VITROS 350 and Beckman Coulter LX20, and data were analyzed by statistical methods. Results The reference intervals showed no significant difference between boys and girls except CK on the dry and Wet chemistry analyzer respectively（P〉0.05）. But the results of 6 serum enzyme items showed significant difference between the VITROS 350 and Beckman Coulter LX20（P〈0.05）. Conclusion The results showed significant difference between the VITROS 350 and Beckman Coulter LX20, the reference intervals of 6 serum enzyme items on the dry and wet chemistry analyzer among healthy children aged from 3 to 6 years in Shenzhen should be established according to different methods.

关 键 词：血液化学分析 参考值 儿童 深圳 

分 类 号：R446.1[医药卫生—诊断学]