番茄黄化曲叶病毒山东泰安分离物的全基因组克隆与分析  

Cloning and Sequence Analysis of Taian Isolate of Tomato Yellow Leaf Curl Virus in Shandong Province

作　　者：刘文浩[1] 仇平平[1] 辛相启[2] 苏文敏[1] 王祥[1] 竺晓平[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]山东省农业科学院植物保护研究所,山东济南251000

出　　处：《山东农业科学》2012年第11期4-7,共4页Shandong Agricultural Sciences

基　　金：国家公益性行业科研专项(201003065)资助

摘　　要：2011年春季在山东泰安保护地采集疑似番茄黄化曲叶病毒病症状的样品(SDTA),利用双生病毒的通用引物及DNA-A基因的全长引物对样品进行扩增并测序,得到共2 781 bp的核苷酸序列。序列比对发现,该样品与番茄黄化曲叶病毒安徽分离物(FN650807)同源性最高为99.6%,系统进化树表明,该样品是番茄黄化曲叶病毒,属于番茄黄化曲叶病毒以色列株系。The samples （SDTA） showing typical symptoms of tomato yellow leaf curl disease were collected from protected tomato planting field of Taian, Shandong Province in spring of 2011. In order to identify the causal virus, the universal primers and the primers for full - length DNA - A gene amplification of geminivirus were used to amplify the sample, and a 2 781 bp fragment of the virus complete genome was obtained. The sequence analysis showed the complete genomic sequence of SDTA had the highest identity of 99.6% with tomato yellow leaf curl virus isolate of Anhui （ FN650807）, and the phylogenic analysis results indicated that SDTA belonged to Israel strain of the tomato yellow leaf curl virus.

关 键 词：番茄黄化曲叶病毒 DNA—A 序列分析 

分 类 号：Q781[生物学—分子生物学]