千代田锦的组培快繁技术  

Study on Tissue Culture and Rapid Propagation Technique of Aloe variegata

作　　者：骆震[1] 王明友[1] 张红[1] 

机构地区：[1]德州学院农学系,山东德州253023

出　　处：《山东农业科学》2012年第11期52-54,共3页Shandong Agricultural Sciences

基　　金：德州市科技发展计划项目(20080153)

摘　　要：以千代田锦一年生健壮幼芽为外植体,MS为基本培养基,探索了千代田锦的组培快繁技术,为其工厂化生产提供依据。结果表明:最佳启动培养基为MS+2.0 mg/L 6-BA+0.1 mg/L NAA,接种40 d后即可诱导出芽;增殖培养以MS+2.0 mg/L 6-BA+0.5 mg/L NAA较适宜;根的诱导以1/2MS(大量元素减半)+0.5 mg/L NAA+0.5 mg/L IBA为其最适培养基。To provide theoretical guidance for the industrial production of Aloe variegate, its tissue culture and rapid propagation technologies were explored with the haleness plumules as explants and MS as basic medium. The results indicated that the optimum startup culture medium was MS ＋ 2.0 mg/L 6 - BA ＋ 0.1 mg/L NAA, and the buds could be induced after 40 days. MS ＋ 2.0 mg/L 6 - BA ＋ 0.5 mg/L NAA was better for multiplication, and 1/2MS （major elements was halved） ＋ 0.5 mg/L NAA ＋0.5 mg/L IBA was the optimum one for rooting culture.

关 键 词：千代田锦 组培快繁 

分 类 号：S682.360.47[农业科学—观赏园艺]