96-Ⅱ喜树叶片高频率植株再生体系建立  

Establishment of A Highly Efficient Regeneration System for Leaf Pieces of 96-Ⅱ Camptotheca acuminate

作　　者：董凤丽[1] 祖元刚[2] 王慧梅[2] 

机构地区：[1]黑龙江生态工程职业学院,哈尔滨150025 [2]森林植物生态学教育部重点实验室(东北林业大学)

出　　处：《东北林业大学学报》2012年第12期56-59,共4页Journal of Northeast Forestry University

基　　金：国家重点基础研究规划项目(G19990160)

摘　　要：用96-Ⅱ喜树无菌苗叶片为外植体,筛选出了96-Ⅱ喜树最佳愈伤组织诱导培养基为WPM+6-BA1.0 mg/L+NAA0.5 mg/L+2,4-D0.1 mg/L,愈伤组织诱导率95%以上;愈伤组织分化培养基为WPM+6-BA0.5 mg/L,分化率达90%;最佳生根培养基为WPM+IBA0.8 mg/L,生根率为94%以上,平均根数为4～8条;移栽成活率达到95%以上。Adventitio buds were induced using leaf discs from the sterile seedlings of 96-Ⅱ Camptotheca acuminata as explants and cultured on WPM, MS and 135 culture media supplemented with various concentrations of hormones 6-BA, NAA and 2,4-D. Results showed that WPM containing 1.0 mg/L 6-BA, 0.5 mg/L NAA and 0. I mg/L 2,4-D was the optimal me- dium for callus induction, with a callus induction frequency of 95%. WPM supplemented with 0.5 mg/L 6-BA was the best medium for the callus differentiation, with a differentiation frequency of 90%. WPM with 0.8 mg/L IBA was the optimal medium for rooting. Four to eight roots appeared and the rooting rate was up to 94%. The survival rate of transplants reached 95%.

关 键 词：96-Ⅱ喜树 叶片 植株再生 

分 类 号：Q943.1[生物学—植物学]