贵州省侗族、汉族人群乙型肝炎病毒感染结局与趋化因子RANTES基因-403G／A及-28C／G位点多态性研究  

Study on the association between RANTES-403GIA as well as -28C/G gene polymorphism and their susceptibility to the hepatitis B virus infections in Dong and Han ethnicities in Guizhou,China

作　　者：张婵[1] 单可人[2] 何燕[2] 王婵娟[2] 张婷[2] 李毅[2] 邓婕[2] 李林洁[2] 欧阳凯[2] 官志忠[2] 

机构地区：[1]洛阳市中心医院生殖助孕中心,471002 [2]贵阳医学院分子生物重点实验室

出　　处：《中华流行病学杂志》2012年第12期1279-1282,共4页Chinese Journal of Epidemiology

基　　金：贵州省科技计划（黔科合SY[2010]3001、LG[2011]024）

摘　　要：目的探讨贵州省世居侗族和汉族人群趋化因子RANTES基因（regulated upon activation normal T cell expressed and secreted）-403G／A和-28C／G位点多态性与乙型肝炎病毒（HBV）感染后结局的相关性。方法采取病例对照方法，募集侗族和汉族共计229例HBV持续感染者、161例HBV清除者作为研究对象，同时选取200例正常者作为对照组。应用TaqMan—MGB探针实时荧光聚合酶链反应SNP分型技术对趋化因子RANTES基因-403G／A和-28C／G位点进行分型测定。结果趋化因子RANTES基因-403G／A和-28C／G基因型频率在侗族汉族HBV持续感染组、HBV清除组和正常对照组间差异无统计学意义（P〉0．05），单因素分析发现-403AG和-28GG增加HBV持续感染的危险度（OR=1．292，OR=1．151）。趋化因子RANTES-28C／G基因型频率在侗族和汉族HBV持续感染组分布差异有统计学意义（P〈0．05），而RANTES-403G／A各基因型在两族人群中分布的差异无统计学意义（P〉0．05）。结论携带趋化因子RANTES-403AG和-28GG基因型HBV持续感染风险性高。RANTES-403A有助于HBV清除。Objective To investigate the association between both regulated upon activation normal T cell expressed and secreted （RANTES）-403G/A, -28C/G gene polymorphism and the susceptibilities to hepatitis B virus （HBV） infection, among people with Dong and Han ethnicities, in Guizhou. Methods A total of 229 individuals with HBV persistence infection, 161 HBV clearanced patients and another 200 controls were recruited to conduct a case-control study among residents with Dong or Han ethnicities. Allelic frequencies of both RANTES-403G/A and -28C/G were identified by TaqMan-MGB probe. Results Both RANTES-403G/A and-28C/G polymorphism in the HBV- persistent group, when compared to the HBV-clearances group, no significant difference was found （P〉0.05）. Results from the univariate analysis showed that subjects carrying-403AG and-28GG genotype had higher risk on the susceptibility to HBV persistence infection. The distributions of RANTES-28C/G gene polymorphism between Dong minority and Hart ethnicities regarding HBV persistence showed statistically significant difference （P〈0.05）. There was no difference on the distributions of RANTES-403G/A gene polymorphism between Dong minority and Hart ethnicities. Conclusion Patients that carrying both RANTES-403AG and-28GG genotype had higher risk on the persistence to HBV, while RANTES-403A had contributed to the clearance of HBV infection.

关 键 词：乙型肝炎病毒 趋化因子RANTES 基因多态性 

分 类 号：R51[医药卫生—内科学]