谷胱甘肽修饰的CdTe量子点共振光散射法测定溶菌酶及其分析应用  

Resonance light scattering method for the determination of lysozyme using glutathione-capped CdTe quantum dots and its analytical application

作　　者：董微[1] 王莹[2] 宋有涛[2] 徐淑坤[3] 葛欣[1] 

机构地区：[1]沈阳医学院化学教研室,辽宁沈阳110034 [2]辽宁大学生命科学院,辽宁沈阳110036 [3]东北大学化学系,辽宁沈阳110819

出　　处：《化学试剂》2012年第12期1093-1095,共3页Chemical Reagents

基　　金：国家自然科学基金资助项目(20875011);辽宁省自然基金资助项目(20102221);辽宁省教育厅资助项目(L2010542);辽宁省教育厅杰出青年学者成长计划资助项目(LJQ2011109);沈阳市科技局资助项目(F11-262-9-21)

摘　　要：以谷胱甘肽(GSH)为稳定剂,在水溶液中制备稳定的CdTe纳米量子点。基于溶菌酶在pH 7.4的磷酸盐缓冲液中对该量子点的共振散射有增强作用,建立一种简便灵敏的测定溶菌酶的方法。考察了缓冲液pH、量子点的浓度和反应时间等对溶菌酶测定的影响。结果表明,量子点在波长422 nm处的散射光强度的增强与溶菌酶浓度呈线性关系,线性范围为1.4～28 mg/L,检出限0.027 mg/L(3σ)。此方法应用于溶菌酶含片中溶菌酶含量的检测,回收率在98.7%～98.9%之间,相对标准偏差<4.2%。CdTe quantum dots （QDs） were synthesized by chosing L-Glutathione （GSH） as modifying agent in aqueous solution. Based on the significant enhancement effect of lysozyme on the resonance light Scattering GSH-CdTe in the phisphate buffer at pH 7.4, a convenient and feasible method for the determination of lysozyme was proposed. The effects of experimental conditions,including pH of the buffer, the concentration of CdTe QDs and the reaction time on the determination of lysozyme were investigated. The results showed the enhanced RLS intensity of CdTe QDs was linear with mass con- centration of lyszome in the range of 1.4 - 28 mg/L as measured at 422 nm, with the detection limit of 0. 027 mg/L （3or）. The method was used to the detection of the lysozyme in lyszome buccal tables samples with the recovery from 98.7% -98.9% and the RSD lower than 4. 2%.

关 键 词：谷胱甘肽修饰的CdTe量子点 共振光散射 溶菌酶 

分 类 号：O657.3[理学—分析化学]