HIV-1感染者中枢神经系统的病毒学特征研究  

Research on virological characteristics in central nervous system of HIV-1 infected patients

作　　者：丁渭[1] 张玉林[1] 乔录新[1] 徐树莹[1] 宋凤丽[1] 陈德喜[1] 

机构地区：[1]首都医科大学附属北京佑安医院北京市肝病研究所,100069

出　　处：《北京医学》2012年第12期1037-1040,1022,共4页Beijing Medical Journal

基　　金：国家自然科学基金(30910103915)

摘　　要：目的探讨HIV-1感染者中枢神经系统(CNS)的病毒学特征。方法通过克隆测序分析病毒亚型,观察CNS中HIV-1变异性和离散率;依据V3环11/25法则并结合10例有偿献血HIV-1感染者尸检脑组织标本免疫荧光试验分析中枢神经HIV-1复合受体的使用。结果有偿献血人群感染的HIV-1显示B和B′亚型特征,脑脊液(CSF)和外周血序列有嵌合现象;CSF C2-V5区序列变异性明显低于外周血,两者的离散率差异无统计学意义;依据11/25法则分析V3环序列可见中枢神经HIV-1大多数使用CCR5复合受体。结论 CNS HIV-1病毒变异性明显小于外周血;CNS HIV-1具有CCR5嗜性。Objective To study the virological characteristics in central nervous system （CNS） of HIV-1 infected patients. Methods Virus subtype was analyzed by cloning and sequencing. Variability and discrete rate of HIV-1 in CNS were observed. Co-receptor usage of HIV-1 in CNS was characterized as according to V3-1oop 11/25 charge rule and fur- ther confirmed through immunoflourescence in autopsies of 10 HIV-1 infected professional blood donors （PBD）. Results HIV-1 from PBDs displayed features of subtypes B＂ and B. Sequence from cerebrospinal fluid （CSF） and peripheral blood had phenomenon of chimerism. C2 - V5 sequence variability in CSF was significantly lower than that in peripheral blood .However, there was no difference in sequence divergency between CNS and blood. Based on V3 loop sequence by the 11/25 rule analysis, most of HIV-1 in CNS used CCR5 coreceptor. Conclusion HIV-1 diversity in CNS is signifi- cantly lower than that in peripheral blood. HIV-1 in CNS has CCR5 tropism.

关 键 词：中枢神经系统 人类免疫缺陷病毒1型 C2-V5区 病毒学特征 

分 类 号：R512.91[医药卫生—内科学]