胶束增敏荧光光度法测定不同蔬菜中草萘胺含量  

Determination of Napropamide in Different Vegetables by Micellar Enhanced Spectrofluorimetry

作　　者：张海容[1] 王雅乐[1] 陈金娥[1] 

机构地区：[1]忻州师范学院生化分析技术研究所,山西忻州034000

出　　处：《分析科学学报》2012年第6期827-830,共4页Journal of Analytical Science

基　　金：山西省国际科技合作项目(No.2010081020);山西省自然科学基金(No.2011011007-1);忻州师范学院2011年教改及特色项目(2011-52-9)

摘　　要：用荧光法探索了曲拉通X-100(Triton X-100)、十二烷基硫酸钠(SDS)、β-环糊精(β-CD)、溴化十六烷基三甲铵(CTMAB)、聚氧乙烯月桂醚(Brij35)5种有序介质,及反应时间、pH条件对草萘胺荧光光谱行为的影响。结果表明,5种有序介质中CT-MAB对草萘胺荧光增敏效果最好,在λex/λem=292/338nm处有强荧光峰。据此,建立了用CTMAB增敏荧光光度法测定草萘胺含量的新方法,方法线性范围为1.0×10-7～1.0×10-4 mol/L,相关系数R=0.9986,检出限为5.3×10-8 mol/L,对1.0×10-4mol/L草萘胺测定的相对标准偏差(RSD)为3.454%。该法已用于西红柿、芹菜、油菜、菠菜、绿尖椒、圆白菜、水萝卜、长茄子、豆角、黄瓜中草萘胺的测定。The effects of reaction time and pH on the fluorescence properties of napropamide in five ordered media such as Triton X-100,SDS, β-CD,CTMAB and Brij35 were investigated by spectrofluorimetry. The result suggested that the fluorescence intensity of napropamide was greatly enhanced in the CTMAB soltuon with a strong peak at λ-ex/λem = 292/338 nm. A linear range of 1. 0 × 10^-7 - 1. 0 × 10^-4 mol/L with the correlation coefficient of R=0. 9986, the detection limit of 5.3 × 10^-8 mol/L,and the RSD of 3. 454% were obtained, respectively. The nanpropaminde contents of 10 kinds of vegetables, such as tomato, celery, rape, spinach, green pepper, cabbage, radish, long eggplant, bean and cucumber, have been successfully determined with a satisfactory result using this method.

关 键 词：草萘胺 溴化十六烷基三甲铵 荧光光度法 胶束增敏 

分 类 号：O657.39[理学—分析化学]