微生物菌体的润湿性和吸附研究  

Wettability and Adsorption Characteristic Study of Microorganisms

作　　者：王萍[1] 夏文杰[1] 罗治斌[2] 董汉平[1] 俞理[1] 黄立信[1] 毕永强[1] 

机构地区：[1]中国科学院渗流流体力学研究所,河北廊坊065007 [2]中国石油勘探开发研究院,北京100030

出　　处：《油田化学》2012年第4期470-473,共4页Oilfield Chemistry

基　　金：国家高技术研究发展计划("863"计划)项目"内源微生物采油技术研究"(项目编号2009AA063504)

摘　　要：在微生物采油中,微生物菌体在多孔介质表面上的吸附是影响微生物提高采收率的因素之一。本文报道了碳源及培养时间对WJ-1菌体表面润湿性和在石英砂表面吸附量的影响。结果表明,随培养时间的延长,以糖蜜为碳源培养菌体的表面亲油性值从36.8%降至16.4%,表现为亲水性,在石英砂表面的吸附效率为68.8%,并使石英砂表面接触角从36.5°降至10.8°;以新疆原油为碳源培养菌体的表面亲油性值从36.8%增加到72.4%,表现为亲油性,在石英砂表面的吸附效率为79.5%,并使石英砂表面接触角从36.5°增至80.2°。物理模拟实验表明,亲水菌体的堵塞调剖作用明显,而亲油菌体的降解作用明显。以新疆原油为碳源,培养120 h后得到的WJ-1菌可使采收率提高4.02%。The absorption of microbes on the surface of porous medium was one of the factors affecting the efficiency of oil recovery in microbial enhanced oil recovery. The effects of carbon source and incubation time on cell surface wettability and absorption of microorganism on quartz sand were studied. The results showed that cell surface lipophilicity and contact angle decreased from 36.8% to 16.4% and from 36.5° to 10.8° with increasing incubation time when the carbon source was molasses, and the adsorption efficiency of cell was of 68.8%. Whereas cell surface lipophilicity and contact angle increased from 36.8% to 72.4% and from 36.5° to 80.2° when the carbon source was Xinjiang crude oil, and the adsorption efficiency of cell was of 79.5%. Physical simulation experiments showed that hydrophilic strain obviously contributed to the profile modification, while hydrophobic strain obviously contributed to the degradation. The oil recovery increased about 4.02% by WJ-1 strain with Xinjiang crude oil carbon source and 120 hrs incubation time.

关 键 词：润湿性 吸附 铜绿假单胞菌 微生物采油 

分 类 号：Q939.97[生物学—微生物学] TE39[石油与天然气工程—油气田开发工程]