黄连-黄芩药对化学成分的UPLC-PDA-MS分析  

Analysis of the Main Components of Coptis-Scute Herbal Pair by UPLC-PDA-MS

作　　者：张晓雷[1,2] 周明眉[1] 赵爱华[3] 苟小军[1] 吴建兵[1] 恽祥惠[1] 邢丽娜[1] 

机构地区：[1]上海中医药大学 [2]海绿谷制药有限公司,上海201203 [3]海交通大学药学院,上海200240

出　　处：《天然产物研究与开发》2012年第11期1502-1507,共6页Natural Product Research and Development

基　　金：上海市科委项目(06DZ05906,06DZ19713);上海市教委创新项目(09YZ119);E研究院(E03008)

摘　　要：本文建立了黄连-黄芩药对化学成分的UPLC-PDA-MS分析方法。实验采用ACQUITY UPLC BEH C18柱,流动相为0.05%甲酸水和甲醇梯度洗脱;检测波长280 nm;MS一级全扫描模式。综合分析标准品及不同样品的色谱峰保留时间、紫外光谱及MS一级全扫描质谱图,归属了黄连-黄芩提取液中的12个主要色谱峰,鉴别出8种成分,推断出2种成分,同时对鉴别出的8种成分进行了定量,被测成分在线性范围内均具有良好的线性关系(r≥0.9991),精密度、重复性的RSD均小于5.0%,加样回收率基本在95%～105%内。本方法快捷、准确,重复性好,能同时测定8个主要化学成分,可较全面地控制黄连-黄芩药对化学成分的含量。In this study, a comprehensive UPLC-PDA-MS method was developed, optimized and validated to investigate the chemical eomponents of Coptis-Scute composite herbal pair. Chromatographie separation was carried out using an Aequity UPLC BEH C18 column eluted with a mobile phase eonsisting of 0.05% formie acid in water and methanol. Twelve main peaks were detected in the chromatogram of Coptis-Scute sample based on the comparison of retention times ,on line UV spectra and MS spectra of the standards and different samples. Eight components were identified and confirmed,two components were tentatively assigned. In addition, the concentrations of the identified eight eomponents were quantified. The relationship between the concentration and the peak areas of the eight compounds was all linear. The RSDs of precision and reproducibility were all less than 5.0%. The average reeovefies was 95% to 105%. The method developed in this study was rapid, simple, reliable and accurate. It had been successfully used for the quantifieation of eight compounds in Coptis-Scute composite herbal pair.

关 键 词：黄连 黄芩 配伍 UPLC-PDA-MS 

分 类 号：R917[医药卫生—药物分析学]