松针叶绿素-胡萝卜素软膏的体外抗甲型流感病毒作用研究  

Experimental study on effect of chlorophyll-carotene ointment from pine needles against influenza A virus in vitro

作　　者：郑光耀[1] 何玲[2] 李娇[3] 薄采颖[1] 李海涛[3] 周涛涛[2] 

机构地区：[1]中国林业科学研究院林产化学工业研究所国家林业局林产化学工程重点开放性实验室生物质化学利用国家工程实验室,江苏南京210042 [2]中国药科大学药理学教研室,江苏南京210009 [3]南京中医药大学药学院,江苏南京210046

出　　处：《华西药学杂志》2012年第6期641-643,共3页West China Journal of Pharmaceutical Sciences

基　　金：科技部农业科技成果转化资金项目(2008GB24320412);2010年教育部"新世纪优秀人才"支持计划资助项目(NCET-10-0817);国家"十二五"重大新药创制专项(2012ZX09504001-001)

摘　　要：目的观察松针叶绿素-胡萝卜素软膏的体外抗甲型流感病毒作用。方法接种甲型流感病毒于狗肾传代细胞(MD-CK),评价松针叶绿素-胡萝卜素软膏的细胞毒性及体外抗病毒作用。结果松针叶绿素-胡萝卜素软膏对MDCK细胞的半数中毒浓度(TC50)为56μg·mL-1,治疗和预防给药方式均有抗甲型流感病毒作用,半数抑制浓度(IC50)分别为30、29μg·mL-1,治疗指数(TI)均为1.9。结论松针叶绿素-胡萝卜素软膏具有明显的抗流感病毒作用,且在安全浓度范围内呈现一定的量效关系,浓度越高抗病毒作用增强。OBJECTIVE To investigate the anti-influenza A virus activity of chlorophyll-carotene ointment from pine needles in vitro.METHODS Influenza A virus into Madin-Darby canine kidney（MDCK） cells were vaccinated,and the cytotoxicity and anti-virus acivity of chlorophyll-carotene ointment in vitro evaluated.RESULTS The median toxic concentration（TC50） of chlorophyll-carotene ointment on MDCK cells was 56 μg·mL-1.The median inhibitory concentration（IC50） of therapy and prophylaxis were 30 and 29 μg·mL-1,respectively.The therapeutic index（TI） was 1.9.CONCLUSION The chlorophyll-carotene ointment from pine needles had significant effect on anti-influenza A virus in a certain of dose-effect relationship within the safe range of concentration.The antiviral efficiency of chlorophyll-carotene ointment was improved with the increasing of concentration.

关 键 词：松针 提取物 甲型流感病毒 MDCK细胞 抗病毒作用 

分 类 号：R96[医药卫生—药理学]