兰索拉唑片在人体内的药动学研究  

Pharmacokinetic study of Lansoprazole tablets in the human blood

作　　者：李穆琼[1] 孙飞[2] 兰婷[1] 高俊萍[1] 张东旭[1] 李晓晔[1] 

机构地区：[1]第四军医大学药学系药物化学教研室,陕西西安710032 [2]河南洛阳解放军534医院妇产科,河南洛阳471003

出　　处：《华西药学杂志》2012年第6期684-686,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用RP-HPLC法进行兰索拉唑的血药浓度检测和药动学研究。方法采用Agilent C18色谱柱(250 mm×5mm,5μm),流动相为乙腈-1‰三乙胺水溶液(pH7)(30∶70),流速1.0 mL.min-1,进样量20μL,内标为奥美拉唑。血浆样品经乙酸乙酯提取后,于285 nm处检测。结果 0.05～1.60μg·mL-1兰索拉唑与峰面积比值的线性关系良好(r=0.9997),最低定量限为20 ng.mL-1(S/N>3),日内RSD<7.94%(n=5),日间RSD<9.41%(n=5),提取回收率>95.79%。结论所用方法可用于临床上兰索拉唑片血药浓度的检测及药动学研究。OBJECTIVE To establish an HPLC method to determine the concentration of lansoprazole in human blood and the pharmacokinetics of Lansoprazole.METHODS Agilent C18（250 mm×5 mm,5 μm） was used as analytical column and the mobile phase consisted of acetonitrile-water（pH7）（30：70） at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 285 nm,and the injected sample volume was 20 μL.RESULTS The linear range of extracted Lansoprazole in human blood was 0.05-1.60 μg·mL-1（r=0.9997）.The lowest detectable concentration was 20 ng·mL-1（S/N3）.The recovery was higher than 95.79%.The inter-day RSD and intra-day RSD were less than 7.94% and 9.41%（n=5）,respectively.CONCLUSION This method is applicable to determine the concentration of lansoprazole in human plasma for pharmacokinetic study.

关 键 词：兰索拉唑 高效液相色谱法 药物动力学 

分 类 号：R917[医药卫生—药物分析学]