静电纺丝法制备PBS纳米纤维膜及缓释血小板内生长因子的PBS纳米纤维膜的生物活性评价  

Preparation and Bioactivities of Electrospun PBS Nanofiber Membrane and Hybrid PBS Nanofiber Membrane of Sustained Release Growth Factors in Platelet

作　　者：孙淑芬[1] 李男男[1] 刘敏[1] 刘译聪[1] 张英超[2] 周延民[1] 

机构地区：[1]吉林大学口腔医院,长春130021 [2]吉林大学超分子结构与材料国家重点实验室,长春130012

出　　处：《高等学校化学学报》2012年第12期2827-2832,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:81070855)资助

摘　　要：利用静电纺丝法制备了聚丁二酸丁二醇酯(PBS)纳米纤维膜及PBS和富血小板血浆(PRP)的混合纳米纤维膜.通过扫描电子显微镜、3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)比色法、支架表面细胞荧光染色法、材料溶血行为及Elisa法检测体外释放综合评价了支架材料的性能及生物活性.结果表明,纳米纤维膜对人骨肉瘤细胞系MG63细胞增殖具有促进作用;生长因子在最初的突释后,随材料降解而缓释;支架材料溶血率为0.1475%(<5%),符合医用材料的溶血实验要求.Poly （ butylene succinate） （PBS） nanofiber membrane and hybrid nanofiber membrane of PBS and platelet-rich plasma（PRP） were prepared by electrospinning. The properties and bioactivities of scaffold materials were evaluated by means of scanning electron microscope （SEM）, methyl thiazolyl tetrazolium （MTT）, fluorescent staining and laser scanning co-focal microscope, hemolytic properties of materials and enzymelinked immunosorbent assay（ELISA）. The experiment results show that nanofiber membranes can accelerate the proliferation of human osteosarcoma MG63 cells line. Also, in vitro the growth factors in the scaffold material display a sustained release with the scaffold degrading after the initial burst release. In addition, hemolytic rate of scaffold material is 0. 1475% （ 〈5% ）, which meets the demand of hemolytic rate of medial materials.

关 键 词：纳米纤维膜 聚丁二酸丁二醇酯 生长因子 富血小板血浆 缓释 

分 类 号：O635[理学—高分子化学]