生长抑素对急性肠梗阻大鼠肠上皮细胞及外周血淋巴细胞的影响  

Effect of somatostatin on the intestinal epithelial cell and peripheral blood lymphocyte in the acute intestinal obstruction rats

作　　者：郭勇[1] 吕洋 孙璐璐[1] 陈志峰[1] 周毅[3] 龚建平[3] 

机构地区：[1]上海交通大学医学院附属第九人民医院SICU,上海200011 [2]荆门市第二人民医院外科,湖北荆门448000 [3]华中科技大学同济医学院附属同济医院外科,湖北武汉430030

出　　处：《中国急救医学》2012年第12期1113-1116,共4页Chinese Journal of Critical Care Medicine

基　　金：生命科学部主任基金（30440024）;自然科学基金面上项目（39670365）

摘　　要：目的观察生长抑素对急性肠梗阻大鼠肠上皮细胞和外周血淋巴细胞的细胞周期及调亡的影响，并研究生长抑素对肠黏膜屏障的保护功能及减轻肠道炎症反应的效应，阐述其细胞生物学基础。方法将96只大鼠随机分为正常对照组、假手术组、肠梗阻组和生长抑素组。肠梗阻组和生长抑素组采用贯穿缝扎末端回肠肠管的方法建立大鼠不完全性小肠梗阻模型。生长抑素组给予皮下注射生长抑素处理。于手术后24、48及72h分别采样小肠上皮细胞和血淋巴细胞，应用流式细胞仪通过SubGl法检测细胞周期和细胞凋亡。结果生长抑素组的肠黏膜上皮细胞和外周血淋巴细胞的G0-G1期细胞比例较肠梗阻组增加，生长抑素组的肠上皮细胞和外周血淋巴细胞的调亡率较肠梗阻组降低。结论生长抑素可抑制急性肠梗阻大鼠肠上皮黏膜细胞和血淋巴细胞增殖和调亡，从而保护肠道黏膜，并调节免疫。Objective To observe the effect of somatostatin on the cell cycle and apoptosis of the intestinal epithelial cell and peripheral blood lymphocyte in the acute intestinal obstruction rats, to study the effects of somatostatin on protecting intestinal mucosa barrier function and reducing intestinal inflammatory reaction, and to expound the principles of cell biology. Methods 96 rats were randomly divided into normal control group, sham operation group, intestinal obstruction and somatostatin group. In intestinal obstruction and somatostatin group, the rat models of incomplete intestinal obstruction were established by transfixing suture of terminal ileum loop. Somatostatin group were given subcutaneous injection of somatostatin treatment. At 24, 48 and 72 h after the operation, small intestinal epithelial cells and lymphocytes were respectively harvested, and the cell cycle and apoptosis were detected with the application of flow cytometry by the SubG1 method. Results The GO - G1 cell ratio of intestinal epithelial cells and peripheral blood lymphocytes was higher in somatostatin group than in intestinal obstruction group, and the apoptosis rate of intestinal epithelial ceils and peripheral blood lymphocytes was lower in somatostatin group than in intestinal obstruction group. Conclusion Somatostatin can inhibit the proliferation and apoptosis of intestinal epithelial cell and mucosal lymphocytes in acute intestinal obstruction rat, which can protect the intestinal mucosa and modulate the immune.

关 键 词：生长抑素 急性肠梗阻 肠上皮细胞 外周血淋巴细胞 细胞凋亡 

分 类 号：R593.241[医药卫生—内科学]