响应面分析法优选大蓟炮制工艺  

Application of Response Surface Methodology in Optimization of the Processing Technology of Cirsium japonicum DC

作　　者：余香[1] 龚千锋[1] 叶耀辉[1] 陈泣[1] 聂小忠[2] 易炳学[1] 

机构地区：[1]江西中医学院药学院,南昌330004 [2]深圳职业技术学院,广东深圳518055

出　　处：《中国药学杂志》2012年第24期1983-1986,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81060343)

摘　　要：目的利用响应面分析法对大蓟炮制工艺进行优化。方法在单因素实验基础上选取实验因素与水平,根据中心组合(Box-Benhnken)实验设计原理采用三因素三水平的响应面分析法,确定各工艺条件的影响因素,以HPLC测定大蓟黄酮的主要成分变化为响应面和等高线,优选出大蓟炒炭的最佳工艺。结果大蓟炮制的最佳工艺为炮制时间13 min、炮制温度(310±10)℃、投药量100 g。结论响应面方法是一种有效的统计技术,它是利用实验数据,通过建立数学模型来解决受多种因素影响的最优组合问题,应用于中药炮制工艺优选方面非常有意义。OBJECTIVE To optimize the processing technology of Cirsium japonicum DC by response surface methodology （RSM）. METHODS Based on single factor studies, a three-variable, three-level Box-Benhnken experimental design was used to monitor the effects of independent variables like time, temperature, and weight on the dependent variable, yield of flavonoids when Cir- sium japonieum DC were processed. Response surface and contour plots with flavonoids＇ yield as a function were obtained with the help of HPLC. RESULTS The optimum conditions were processing time 13 min, processing temperature （310 ±10） ℃, and raw material weight 100 g. CONCLUSION RSM is an effective technology to optimize the processing traditional Chinese medicine.

关 键 词：大蓟 炮制工艺 响应面法 

分 类 号：R28[医药卫生—中药学]