红车轴草异黄酮对雌激素缺乏诱发大鼠骨质疏松症的作用研究  

Effect of Trifolium pratense Isoflavones on Osteoporosis Rats Induced by Estrogen Deficiency

作　　者：张小敏[1] 王一林[1] 范文岩[1] 张东明[1] 王作营[2] 周延萌[1] 

机构地区：[1]泰山医学院药学院,山东泰安271016 [2]新汶矿业集团中心医院,山东泰安271219

出　　处：《中国药学杂志》2012年第24期2000-2003,共4页Chinese Pharmaceutical Journal

基　　金：山东省高等学校科技计划项目(J11LF77);泰安市大学生科技创新计划(2010D2031)

摘　　要：目的观察红车轴草异黄酮对去卵巢骨质疏松大鼠体内雌激素和骨代谢生化指标的影响,初步探讨其作用机制。方法取60只6月龄雌性Wistar大鼠随机分为假手术组和5个去卵巢组:模型组、雌激素组(2.5 mg.kg-1)和红车轴草异黄酮高(90 mg.kg-1)、中(60 mg.kg-1)、低(30 mg.kg-1)剂量组,给药12周后处死大鼠,取血液、尿液进行相关生化指标的检测。结果红车轴草异黄酮对去卵巢导致的体重增加有对抗作用;显著降低骨质疏松大鼠血清碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)和骨钙素(BGP)水平,升高体内雌激素水平;显著降低尿钙、尿磷含量。结论红车轴草异黄酮对骨质疏松大鼠有防治作用,其机制可能与提高体内雌激素水平,抑制骨吸收,促进骨形成有关。OBJECTIVE To examine the effects of Trifolium pratense isoflavones（TPIF） , the main active component of Trifolium pratense L. , on ovariectomy-induced osteoporosis in rats. METHODS A total of 60 six-month-old female rats were randomly assigned to a sham-operated group and five ovariectomized（OVX）groups, ie, OVX with vehicle （10 mL · kg^-1 . d^-1, ig）, OVX with graded doses of TPIF（30, 60, and 90 mg · kg ^-1· d^-1 , ig）, and OVX with nilestriol（2. 5 mg· kg^-1 ·week^-1 , ig）. The animals were sacrificed after 12-week treatment. RESULTS Compared to the OVX group, TPIF significantly inhibited the increase of body weight. In addition, TPIF administration significantly decreased serum levels of alkaline phosphatase（ALP） and tartrate-resistant acid phosphatase （TRAP）, and increased serum level of estrogen. CONCLUSION TPIF may be a potential alternative medicine for the prevention and treatment of postmenopausal osteoporosis. Its mechanism may be related to increasing estrogen level, inhibiting bone resorption, and promoting bone formation.

关 键 词：红车轴草异黄酮 骨质疏松 去卵巢大鼠 雌激素缺乏 

分 类 号：R965[医药卫生—药理学]