LC-MS/MS同时测定卷烟主流烟气中4种芳香胺  

Simultaneous Determination of Four Aromatic Amines in Mainstream Cigarette Smoke with Liquid Chromatography Tandem Mass Spectrometry

作　　者：余晶晶[1] 王昇[1] 谢复炜[1] 陈玉松[1] 赵阁[1] 张晓兵[1] 

机构地区：[1]中国烟草总公司郑州烟草研究院

出　　处：《烟草科技》2012年第12期44-48,共5页Tobacco Science & Technology

基　　金：国家自然科学基金项目"卷烟烟气中主要杂环胺类化合物的体内代谢研究"(21005087);中国烟草总公司郑州烟草研究院院长科技发展基金项目"高效液相色谱-串联质谱法检测卷烟主流烟气中的芳香胺"(312010CA0220)

摘　　要：建立了测定卷烟主流烟气中4种主要芳香胺(1-氨基萘、2-氨基萘、3-氨基联苯和4-氨基联苯)的液相色谱-串联质谱(LC-MS/MS)方法,对国内外不同焦油含量卷烟烟气中4种芳香胺的含量进行了检测,并与GC/MS方法进行了比较。结果表明:①4种芳香胺的检测限在0.016～0.038 ng/mL之间,方法回收率在70.5%～116.5%之间,日内精密度小于6%,日间精密度小于9%;②与GC/MS测定结果相比较,二者的相关性较好。该方法快速、灵敏、操作简单,适合于卷烟主流烟气中4种芳香胺的同时测定。For simultaneous determination of four aromatic amines, including 1-naphthylamine, 2-naphthylamine, 3-aminobiphenyl and 4-aminobiphenyl, in mainstream cigarette smoke, a liquid chromatography tandem mass spectrometric （LC-MS/MS） method was developed. The contents of the four aromatic amines in mainstream smoke of domestic and foreign cigarettes with different tar deliveries were determined with the developed method, and the resultant were compared with that determined with GC-MS method. The results showed that： 1） The limits of detection of LC-MS/ MS method ranged from 0.016 to 0.038 ng/mL with the recoveries from 70.5% to 116.5%, and the intra-day and inter-day precisions were less than 6% and 9%, respectively. 2） The resultant of LC-MS/MS method well correlated with that of GC-MS method. The developed method was fast, simple, sensitive and suitable for the simultaneous determination of the said aromatic amines in mainstream cigarette smoke.

关 键 词：LC—MS MS 主流烟气 芳香胺 

分 类 号：TS411.2[农业科学—烟草工业]