我国烟区病毒病种类的血清学鉴定  

Serological Identification of Viruses Infecting Tobacco in Major Tobacco Growing Areas of China

作　　者：罗朝鹏[1] 杨军[1] 王燃[1] 李峰[1] 金立锋[1] 魏春阳[1] 林福呈[1] 

机构地区：[1]中国烟草总公司郑州烟草研究院

出　　处：《烟草科技》2012年第12期72-77,共6页Tobacco Science & Technology

基　　金：郑州烟草研究院科技项目"烟草主要病毒病分子变异监控及追踪技术研究"(122009CZ0410);院长科技发展基金项目"烟草香气前体物代谢途径关键酶基因DXS克隆及表达特征研究"(902011CA0330)

摘　　要：为了全面掌握我国烟草病毒病的发生现状,于2010和2011年连续两年对全国13个主要烟区共409份病毒病样品进行了烟草普通花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaicvirus,CMV)、马铃薯Y病毒(Potato virusY,PVY)和烟草蚀纹病毒(Tobacco etch virus,TEV)ELISA检测。结果表明,TMV侵染率最高,2010年样品侵染率高达64.6%,2011年为48.2%;2010年CMV侵染率为32.1%,2011年为28.2%;PVY侵染率2010年为15.6%,2011年为11.4%;TEV的侵染率最低。共存在7种复合侵染类型,TMV和CMV复合侵染最普遍,2010和2011年样品复合侵染率分别为34.9%和28.6%。说明TMV和CMV是侵染我国烟草的主要病毒,病毒病田间复合侵染较为严重。In order to better understand the occurrence of tobacco virus diseases in China status quo, 409 symptomatic tobacco samples were collected from 13 major tobacco growing areas of China in 2010 and 2011. Tobacco mosaic virus （TMV）, cucumber mosaic virus （CMV）, potato virus Y （PVY） and tobacco etch virus （TEV） were detected by ELISA （enzyme-linked immunosorbent assay）. The results showed that the infection rate of TMV was the highest, which was 64.6% and 48.2% in 2010 and 2011, respectively; that of CMV was 32.1% and 28.2%, respectively; and that of PVY was 15.6% and 11.4%, respectively; while that of TEV was the lowest. There presented 7 types of mixed infection. The mixed infection of TMV＋CMV topped the highest rate of 34.9% and 28.6% in 2010 and 2010, respectively. It indicated that TMV and CMV were the predominant viruses infecting tobacco in China, and the mixed infection occurred seriously in tobacco field.

关 键 词：烟草 病毒 复合侵染 ELISA 

分 类 号：S432.41[农业科学—植物病理学]