傅立叶变换红外光谱技术对两株大肠杆菌的鉴别  

Differentiation of two strains of Escherichia coli by FT-IR spectroscopy

作　　者：王静[1,2] 杨丽君 李兆杰[2] 刘玉敏 时文春 崔凤杰 宋晓华 丛伟红 

机构地区：[1]山东大学威海分校,山东威海264209 [2]威海出入境检验检疫局,山东威海264205 [3]烟台出入境检验检疫局,山东烟台264200

出　　处：《微生物学通报》2012年第12期1844-1850,共7页Microbiology China

基　　金：国家质量监督检验检疫总局科研项目(No.2010IK151;2012IK179)

摘　　要：【目的】应用傅立叶变换红外光谱(Fourier transform infrared spectroscopy,FT-IR)技术对两株不同来源的大肠杆菌进行鉴别。【方法】用FT-IR技术对两株不同来源的大肠杆菌进行指纹图谱数据采集,用化学计量学分析方法对光谱进行分析。【结果】建立了基于主成分分析(Principal component analysis,PCA)和分级聚类分析(Hierarchical cluster analysis,HCA)两种聚类分析模型,均可将两株大肠杆菌进行成功区分。【结论】傅立叶变换红外光谱分析方法简便、快速、易操作,结果重现性好,可用于区分不同来源的同种细菌。[Objective] To differentiate two strains of Escherichia coli from different sources by fourier transform infrared spectroscopy （FT-IR）. [Methods] FT-IR fingerprint absorption spectra of two strains of E. coli from different sources were collected and analyzed by chemom- etric methods. [Results] Two cluster models of principal component analysis （PCA） and hier-archical cluster analysis （HCA） were established and could differentiate well the two strains of E. coli. [Conclusion] As a rapid, easy-to-use and accurate technique, FT-IR spectroscopy can be used to differentiate microorganisms at the strain level from different sources.

关 键 词：傅立叶变换红外光谱 大肠杆菌 株 鉴别 

分 类 号：R115[医药卫生—公共卫生与预防医学]